Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base

Author(s)

Martinez, Diego Stefani T. ^{[1, 2, 3]} ; Da Silva, Gabriela H. ^[3] ; de Medeiros, Aline Maria Z. ^{[1, 2, 3]} ; Khan, Latif U. ^{[2, 3, 4]} ; Papadiamantis, Anastasios G. ^{[2, 5]} ; Lynch, Iseult ^[2]

Affiliation

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, BR-13416000 Piracicaba, SP - Brazil

^[2] Univ Birmingham, Sch Geog Earth & Environm Sci, Birmingham B15 2TT, W Midlands - England

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083100 Campinas, SP - Brazil

^[4] Synchrotron Light Expt Sci & Applicat Middle East, Allan 19252 - Jordan

^[5] NovaMechanics Ltd, CY-1065 Nicosia - Cyprus

• Document type: Journal article
• Source: NANOMATERIALS; v. 10, n. 10 OCT 2020.
• Web of Science Citations: 0

Abstract

In this work, we evaluated the effect of protein corona formation on graphene oxide (GO) mixture toxicity testing (i.e., co-exposure) using the Daphnia magna model and assessing acute toxicity determined as immobilisation. Cadmium (Cd2+) and bovine serum albumin (BSA) were selected as co-pollutant and protein model system, respectively. Albumin corona formation on GO dramatically increased its colloidal stability (ca. 60%) and Cd2+ adsorption capacity (ca. 4.5 times) in reconstituted water (Daphnia medium). The acute toxicity values (48 h-EC50) observed were 0.18 mg L-1 for Cd2+-only and 0.29 and 0.61 mg L-1 following co-exposure of Cd2+ with GO and BSA@GO materials, respectively, at a fixed non-toxic concentration of 1.0 mg L-1. After coronation of GO with BSA, a reduction in cadmium toxicity of 110 % and 238% was achieved when compared to bare GO and Cd2+-only, respectively. Integration of datasets associated with graphene-based materials, heavy metals and mixture toxicity is essential to enable re-use of the data and facilitate nanoinformatics approaches for design of safer nanomaterials for water quality monitoring and remediation technologies. Hence, all data from this work were annotated and integrated into the NanoCommons Knowledge Base, connecting the experimental data to nanoinformatics platforms under the FAIR data principles and making them interoperable with similar datasets. (AU)

• FAPESP's process: 19/07058-0 - Nanotoxicity testing and informatics: harmonization of methodologies for environmental nanosafety and regulation
• Grantee: Diego Stéfani Teodoro Martinez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/25140-3 - Environmental Nanoscience - Understanding the impacts of nanomaterial eco-corona formation on toxicity and working towards a Brazil-EU nanosafety research e-infrastructure
• Grantee: Diego Stéfani Teodoro Martinez
• Support Opportunities: Scholarships abroad - Research