Application of Dispersive Liquid-Liquid Microextraction Followed by High-Performance Liquid Chromatography/Tandem Mass Spectrometry Analysis to Determine Tetrabromobisphenol A in Complex Matrices

Author(s)

Macedo, Williane Vieira ^[1] ; Bernegossi, Aline Christine ^[2] ; Sabatini, Carolina Aparecida ^[1] ; Corbi, Juliano Jose ^[2] ; Zaiat, Marcelo ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Sch Engn, Biol Proc Lab, Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul Engn & Sanitat, Aquat Ecol Lab, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Environmental Toxicology and Chemistry; v. 39, n. 11, p. 2147-2157, NOV 2020.
• Web of Science Citations: 0

Abstract

An accurate and sensitive ultrasound-dispersive liquid-liquid microextraction technique followed by high-performance liquid chromatography separation coupled with electrospray ionization tandem mass spectrometry detection method to determine the presence of tetrabromobisphenol A (TBBPA) in complex environmental matrices is proposed. The miniaturized procedure was used to extract and quantify the analyte in domestic sewage, anaerobic sludge, and the aquatic test organism speciesDaphnia magnaandChironomus sancticaroli, which are standardized organisms for ecotoxicity bioassays. Limits of detection of 2 ng L-1(domestic sewage), 2 ng g(-1)(anaerobic sludge), 0.25 ng g(-1)(D. magna), and 5 ng g(-1)(C. tentans) were obtained. The presence of TBBPA was determined in domestic sewage and anaerobic sludge from an anaerobic batch bioreactor at a concentration of 0.2 +/- 0.03 mu g L(-1)and 507 +/- 79 ng g(-1), respectively. InD. magnaandC. sancticaroliexposed to TBBPA in an acute toxicity bioassay, the micropollutant accumulated at 3.74 and 8.87 mu g g(-1), respectively. The proposed method is a simple and cost-effective tool to determine TBBPA environmental occurrence and biomagnification potential compared with conventional extraction methods. To the best of our knowledge, this is the first liquid-liquid miniaturized extraction method to be applied toD. magnaandC. sancticaroli. Environ Toxicol Chem2020;00:1-11. (c) 2020 SETAC (AU)

• FAPESP's process: 18/17744-6 - Combined system for the treatment of Tetrabromobisphenol a (TBBPA) by anaerobic digestion and advanced oxidative processes
• Grantee: Williane Vieira Macêdo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants