L-Asparaginase Encapsulation into Asymmetric Permeable Polymersomes

Author(s)

Bueno, Cecilia Z. ^{[1, 2]} ; Apolinario, Alexsandra C. ^{[1, 2]} ; Duro-Castano, Aroa ^[2] ; Poma, Alessandro ^{[2, 3]} ; Pessoa, Jr., Adalberto ^[1] ; Rangel-Yagui, Carlota O. ^[1] ; Battaglia, Giuseppe ^{[2, 4, 5]}

Affiliation

^[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil

^[2] UCL, Dept Chem, London WC1H 0AJ - England

^[3] UCL, Eastman Dent Inst, London WC1H 0AJ - England

^[4] Catalan Inst Res & Adv Studies ICREA, Barcelona 08010 - Spain

^[5] Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Barcelona 08028 - Spain

• Document type: Journal article
• Source: ACS MACRO LETTERS; v. 9, n. 10, p. 1471-1477, OCT 20 2020.
• Web of Science Citations: 0

Abstract

This work reports, for the encapsulation of L-asparaginase, an anticancer enzyme into hybrid PMPC25-PDPA(70)/PEO16-PBO22 asymmetric polymersomes previously developed by our group, with loading capacities with over 800 molecules per vesicle. Enzyme-loaded polymersomes show permeability and capacity to hydrolyze L-asparagine, which is essential to cancer cells. The nanoreactors proposed in this work can be potentially used in further studies to develop novel therapeutic alternatives based on Lasparaginase. (AU)

• FAPESP's process: 17/03811-0 - Encapsulation of L-asparaginase in polymersomes by pH-switch method and electroporation
• Grantee: Alexsandra Conceição Apolinário
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/16221-4 - Development of polymersomes permeable to L-asparagine for encapsulation of L-asparaginase
• Grantee: Cecilia Zorzi Bueno
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/05272-0 - Development of porous polymersomes with amphotericin B channels for L-asparaginase encapsulation
• Grantee: Cecilia Zorzi Bueno
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 13/08617-7 - Production of extracellular L-asparaginase: from bioprospecting to the engineering of an antileukemic biopharmaceutical
• Grantee: Adalberto Pessoa Junior
• Support Opportunities: Research Projects - Thematic Grants