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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Experimental dentin adhesives containing calcium orthophosphate particles: Effect on dentin bond strength, micro-permeability and collagen degradation

Full text
Author(s):
Chiari, Marina D. S. [1] ; Alania, Yvette [2] ; Bedran-Russo, Ana K. [2] ; Braga, Roberto R. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Biomat & Oral Biol, Sch Dent, Av Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil
[2] Marquette Univ, Dept Gen Dent Sci, Milwaukee, WI 53233 - USA
Total Affiliations: 2
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES; v. 107, JUN 2021.
Web of Science Citations: 0
Abstract

Purpose: To assess the effect of dicalcium phosphate dihydrate (DCPD) or mineral trioxide aggregate (MTA) particles on the microtensile bond strength (mu TBS), micro-permeability and enzymatic activity at the adhesive-dentin interface of experimental dental adhesives. Materials and methods: An experimental ethanol-based, two-step etch-and-rinse adhesive system was prepared. Four experimental groups were tested, based on the type of particle added: (1) DCPD functionalized with diethylene glycol dimethacrylate (DCPD\_DEGDMA), (2) citric acid (DCPD\_CA), (3) calcium silicate (MTA) and (4) unfilled (control). All particles were added at 10 wt%. Human third molars had their occlusal enamel removed and dentin surface polished. After acid etching, the adhesives were applied on the dentin surfaces and the teeth were restored with a commercial nanofilled resin composite. The mu TBS and micro-permeability were tested after 24 h or 2 months of storage in simulated body fluid (SBF) at 37 degrees C. Bonded interfaces were observed using scanning electron microcopy (SEM). Gelatinolytic activity at the adhesive interfaces was assessed by in situ zymography at 24 h and 2 months in SBF/37 degrees C. The results were described as relative florescent units (RFU). mu TBS data were analyzed by ANOVA/Games-Howell test. Data from micro-permeability and in situ zymography were analyzed by Kruskal-Wallis/Mann-Whitney test (alpha: 5%). Results: Differences in mu TBS were found among adhesives (control = MTA > DCPD\_CA > DCPD\_DEGDMA, p < 0.001), but not between storage times. The control group showed the lowest permeability at both observation periods. Except for DCPD\_CA, no significant increases in micro-permeability were observed after 2 months. All groups presented significant reductions in RFU values between 24 h and 2 months. DCPD\_DEGDMA (at 24 h) and MTA (at 2 months) presented statistically lower RFU than the control (p < 0.01). Conclusion: Evidence of reduced MMP activity was observed at adhesive interfaces with the use of experimental adhesives containing calcium-releasing particles. However, after two months, bond strength and micro-permeability results suggest that interfacial stability was not affected by adhesive formulation. (AU)

FAPESP's process: 16/25971-7 - Development and application of calcium phosphate nanoparticles functionalized with acidic monomers for dental remineralization
Grantee:Marina Damasceno e Souza de Carvalho Chiari
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/02403-9 - Effect on bond strength and collagen degradation of experimental dentin adhesives containing calcium orthophosphate particles
Grantee:Marina Damasceno e Souza de Carvalho Chiari
Support Opportunities: Scholarships abroad - Research Internship - Master's degree