Effect of adrenaline and noradrenaline on biofilm formation and virulence factors of Streptococcus mutans UA159

Author(s)

Cortes, Iago Torres ^{[1, 2]} ; Rosalen, Pedro Luiz ^{[1, 3, 4]} ; Berto, Luciana Aranha ^{[1, 2]} ; Castro, Myrella Lessio ^{[5, 6]} ; Pedrini, Denise Leda ^{[7, 8]} ; Porto, Alessandra Nogueira ^{[7, 9]} ; Cogo-Mueller, Karina ^{[10, 1]} ; Nobre Franco, Gilson Cesar ^{[11, 12]}

Affiliation:Show less -

^[1] Univ Estadual Campinas, Sch Dent Piracicaba, BR-13414903 Piracicaba, SP - Brazil

^[2] Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[3] Univ Fed Alfenas, Grad Program Biol Sci, BR-37130001 Alfenas, MG - Brazil

^[4] Rua Gabriel Monteiro da Silva 700, BR-37130001 Alfenas, MG - Brazil

^[5] Fac Sci Tocantins, BR-77818650 Araguaina, TO - Brazil

^[6] Fac Ciencias Tocantins, Rodovia 222, Km 6, Campus Luis Pires, BR-77818650 Araguaina, TO - Brazil

^[7] Univ Taubate, Taubate, SP - Brazil

^[8] Rua Antonio Paula Nunes 1512, BR-76963784 Cacoal, RO - Brazil

^[9] Ave Ipiranga 316, BR-78032035 Cuiaba, MT - Brazil

^[10] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil

^[11] Univ Estadual Ponta Grossa, Ponta Grossa, PR - Brazil

^[12] Ave Gen Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, Parana - Brazil

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 125, MAY 2021.
• Web of Science Citations: 0

Abstract

Objectives: To evaluate in vitro the effects of adrenaline and noradrenaline on the biofilm formation on orthodontic brackets, acid production and expression of virulence genes of Streptococcus mutans UA159 (S. mutans). Design: S. mutans UA159 biofilm was formed on orthodontic brackets under exposure to adrenaline (100 mu M), noradrenaline (50 mu M) or PBS solution (control group) in triptone-yeast extract with 1 % sucrose. After 24 h, biofilm formation was quantified through Colony Forming Units / mL (CFU/mL) and RNA was extracted to perform gene expression analysis through real-time reverse transcriptase-PCR (RT-qPCR). Evaluation of acid production was carried out on planktonic cultures for 6 h. One-way ANOVA followed by Tukey's test was carried to determine statistical difference. The level of significance was set at 5 %. Results: Catecholamines stimulated biofilm formation of S. mutans in orthodontic brackets (p < 0,05) but did not interfere with acid production (pH reduction) or the expression of the tested genes related to biofilm formation (gtfB, gtfC, gbpA, gbpB, gbpC, gbpD and brpA), aciduric (relA) and acidogenic properties (ldh). Conclusions: The present study was the first to demonstrate that catecholamines can stimulate S. mutans UA159 biofilm formation. These findings can contribute to clarify the role of stress on bacterial metabolism and contribute to the understanding of a possible role on caries development, mainly in orthodontic patients. (AU)

• FAPESP's process: 09/16324-4 - Evaluation of the effect of adrenaline and noradrenaline on the bacterial adhesion (Streptococcus mutans) on orthodontic brackets.
• Grantee: Gilson Cesar Nobre Franco
• Support Opportunities: Regular Research Grants