Reduction of oxidative stress and inflammatory signaling in the commissural nucleus of the solitary tract (commNTS) and rostral ventrolateral medulla (RVLM) in treadmill trained rats

Author(s):Show less -

Farah, Heloisa M. A. T. ^[1] ; Farah, Daniela M. A. T. ^[2] ; Nunes, Jonas ^[2] ; Ogihara, Cristiana A. ^[1] ; Cafarchio, Eduardo M. ^[1] ; Nadiak, Gabriela K. C. ^[1] ; da Conceicao, Rodrigo R. ^[3] ; Giannocco, Gisele ^{[4, 3]} ; De Angelis, Katia ^[5] ; Irigoyen, Maria C. ^[6] ; Farah, Vera M. A. ^[2] ; Sato, Monica A. ^[1]

Affiliation

^[1] Ctr Univ FMABC, Fac Med ABC, Dept Morphol & Physiol, 2000 Lauro Gomes Ave, BR-09060870 Santo Andre, SP - Brazil

^[2] Univ Presb Mackenzie, Dept Cardiovasc & Renal Physiol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Med, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Dept Biol Sci, Diadema, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Physiol, Sao Paulo, SP - Brazil

^[6] Heart Inst, Dept Physiol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Brain Research; v. 1769, OCT 15 2021.
• Web of Science Citations: 0

Abstract

Inflammation has been associated with cardiovascular diseases and the key point is the generation of reactive oxygen species (ROS). Exercise modulates medullary neurons involved in cardiovascular control. We investigated the effect of chronic exercise training (Tr) in treadmill running on gene expression (GE) of ROS and inflammation in commNTS and RVLM neurons. Male Wistar rats (N = 7/group) were submitted to training in a treadmill running (1 h/day, 5 days/wk/10 wks) or maintained sedentary (Sed). Superoxide dismutase (SOD), catalase (CAT), neuroglobin (Ngb), Cytoglobin (Ctb), NADPH oxidase (Nox), cicloxigenase-2 (Cox-2), and neuronal nitric oxide synthase (NOS1) gene expression were evaluated in commNTS and RVLM neurons by qPCR. In RVLM, Tr rats increased Ngb (1.285 +/- 0.03 vs. 0.995 +/- 0.06), Cygb (1.18 +/- 0.02 vs.0.99 +/- 0.06), SOD (1.426 +/- 0.108 vs. 1.00 +/- 0.08), CAT (1.34 +/- 0.09 vs. 1.00 +/- 0.08); and decreased Nox (0.55 +/- 0.146 vs. 1.001 +/- 0.08), Cox-2 (0.335 +/- 0.05 vs. 1.245 +/- 0.02), NOS1 (0.51 +/- 0.08 vs. 1.08 +/- 0.209) GE compared to Sed. In commNTS, Tr rats increased SOD (1.384 +/- 0.13 vs. 0.897 +/- 0.101), CAT GE (1.312 +/- 0.126 vs. 0.891 +/- 0.106) and decreased Cox-2 (0.052 +/- 0.011 vs. 1.06 +/- 0.207) and NOS1 (0.1550 +/- 0.03559 vs. 1.122 +/- 0.26) GE compared to Sed. Therefore, GE of proteins of the inflammatory process reduced while GE of antioxidant proteins increased in the commNTS and RVLM after training, suggesting a decrease in oxidative stress of downstream pathways mediated by nitric oxide. (AU)