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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Congenital Anomalies Programmed by Maternal Diabetes and Obesity on Offspring of Rats

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Araujo-Silva, Vanessa Caruline [1] ; Santos-Silva, Alice [1] ; Lourenco, Andressa Silva [1] ; Barros-Barbosa, Cristielly Maria [1] ; Moraes-Souza, Rafaianne Queiroz [1, 2] ; Soares, Thaigra Sousa [1, 2] ; Karki, Barshana [2] ; Paula, Veronyca Goncalves [1, 2] ; Sinzato, Yuri Karen [2] ; Damasceno, Debora Cristina [2] ; Volpato, Gustavo Tadeu [1]
Total Authors: 11
Affiliation:
[1] Univ Fed Mato Grosso, Inst Biol & Hlth Sci, Lab Syst Physiol & Reprod Toxicol, Barra Do Garcas - Brazil
[2] Sao Paulo State Univ, Lab Expt Res Gynecol & Obstet, Postgrad Program Tocogynecol, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FRONTIERS IN PHYSIOLOGY; v. 12, AUG 10 2021.
Web of Science Citations: 0
Abstract

Embryo-fetal exposure to maternal disorders during intrauterine life programs long-term consequences for the health and illness of offspring. In this study, we evaluated whether mild diabetic rats that were given high-fat/high-sugar (HF/HS) diet presented maternal and fetal changes at term pregnancy. Female rats received citrate buffer (non-diabetic-ND) or streptozotocin (diabetic-D) after birth. According to the oral glucose tolerance test (OGTT), the experimental groups (n = 11 animals/group) were composed of non-diabetic and diabetic receiving standard diet (S) or HF/HS diet. High-fat/high-sugar diet (30% kcal of lard) in chow and water containing 5% sucrose and given 1 month before mating and during pregnancy. During and at the end of pregnancy, obesity and diabetes features were determined. After laparotomy, blood samples, periovarian fat, and uterine content were collected. The diabetic rats presented a higher glycemia and percentage of embryonic losses when compared with the NDS group. Rats DHF/HS presented increased obesogenic index, caloric intake, and periovarian fat weight and reduced gravid uterus weight in relation to the other groups. Besides, this association might lead to the inflammatory process, confirmed by leukocytosis. Obese rats (NDHF/HS and DHF/HS) showed higher triglyceride levels and their offspring with lower fetal weight and ossification sites, indicating intrauterine growth restriction. This finding may contribute to vascular alterations related to long-term hypertensive disorders in adult offspring. The fetuses from diabetic dams showed higher percentages of skeletal abnormalities, and DHF/HS dams still had a higher rate of anomalous fetuses. Thus, maternal diabetes and/or obesity induces maternal metabolic disorders that contribute to affect fetal development and growth. (AU)

FAPESP's process: 16/25207-5 - Evaluation of offspring exposed to mildly diabetic intrauterine millieu, submitted to postnatal hyperlipid diet and treated with a mixture of calcium and vitamin D during pregnancy.
Grantee:Débora Cristina Damasceno
Support Opportunities: Regular Research Grants