Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lack of 5-lipoxygenase in intramembranous and endochondral 129Sv mice skeleton and intramembranous healing

Full text
Simionato, Gustavo Baroni [1] ; da Silva, Ana Claudia Rodrigues [2] ; de Oliva, Andre Hergesel [1] ; Furquim, Elisa Mara de Abreu [3] ; Couto, Maira Cristina Rondina [4] ; Shinohara, Andre Luis [4] ; Biguetti, Claudia Cristina [5] ; Matsumoto, Mariza Akemi [1]
Total Authors: 8
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Prevent & Restorat Dent, Aracatuba, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Diag & Surg, Aracatuba, SP - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[5] Univ Texas Dallas UTD, Dept Bioengn, Dallas, TX - USA
Total Affiliations: 5
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 131, NOV 2021.
Web of Science Citations: 0

Objective: To analyze the lack of 5-lipoxygenase (5LO) on dental socket healing and post-natal phenotype of intramembranous and endochondral bones. Design: Wild type (WT) 129/SvEv (n = 20) and 5LO knockout (5LOKO) (n = 20) male mice underwent tooth extraction of the upper right incisor and were euthanized after 7, 14, and 30 day time points for the evaluation of dental socket healing and histological phenotyping of intramembranous (IM) and endochondral (EC) bones. Microscopic analysis of alveolar sockets included histopathological description, histomorphometry, and immunohistochemistry for 5LO, cyclooxygenase 2 (COX2), and tartrate resistant acid phosphatase (TRAP). Results: Histological phenotyping revealed thicker cortical bone in EC bones (femur and vertebra) of 5LOKO mice compared to WTs, with no differences in collagenous content. Although dental socket healing was similarly observed in both groups, WT mice revealed increased numbers of COX-2+ and 5LO+ cells during bone maturing stage, with a decrease of TRAP+ cells at day 30. On the other hand, an increased quantity of fibroblasts was observed at day 7 in 5LOKO group, as well as increased inflammatory infiltrate and significantly decreased TRAP+ cells at final stages of alveolar socket healing in comparison to WTs. Conclusions: The lack of 5LO in 5LOKO mice resulted in thicker cortical of EC, but not of IM post natal bones. Furthermore, genetic deletion of 5LO in the 5LOKO mice directly affected the inflammatory response during socket healing, influencing initial and late phases of bone repair in a model of post-tooth extraction in 129Sv WT and 5LOKO mice. (AU)

FAPESP's process: 18/19406-0 - Role of 5-lipoxygenase enzyme (5-LO) on the intramembranous and endochondral bone phenotype: comparative macroscopic and microscopic anatomy between 129/Sv-WT and 129/Sv 5-LOKO mice
Grantee:Ana Claudia Rodrigues da Silva
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 19/16067-3 - Influence of nitrogen-containing bisphosphonates (n-BPs) on inflammatory and endocrine factors: basis for the comprehension of osteonecrosis of the jaws related to nBPs pathophysiology
Grantee:Mariza Akemi Matsumoto
Support type: Regular Research Grants
FAPESP's process: 18/14488-9 - Morphological and immunohistochemical aspects of inflammation during dental socket repair in mice presenting osteopetrotic skeletal phenotype treated with nitrogen containing bisphosphonate
Grantee:Gustavo Baroni Simionato
Support type: Scholarships in Brazil - Scientific Initiation