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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantification of airborne SARS-CoV-2 genomic particles in different hospital settings

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Amato-Lourenco, Luis Fernando [1, 2, 3] ; Xavier Costa, Natalia de Souza [1] ; Dantas, Katia Cristina [1] ; Ferreira Spina Lombardi, Suzette Cleuza [4] ; Mendroni Junior, Alfredo [5] ; Lauletta Lindoso, Jose Angelo [6, 7] ; Lima, Felipe Gallego [8] ; Carvalho-Oliveira, Regiani [1] ; Mauad, Thais [1, 2]
Total Authors: 9
[1] Univ Sao Paulo, Fac Med, Dept Pathol, LIM 05, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Adv Studies IEA, Global Cities Program, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med, Dr Arnaldo Ave 455, Room 1150, BR-01246903 Sao Paulo, SP - Brazil
[4] Pro Blood Fdn, Blood Ctr Sao Paulo, Div Res & Transfus Med, Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Dept Hematol, Lab Med Invest Pathogenesis & Target, Sao Paulo - Brazil
[6] Univ Sao Paulo, Dept Molestias Infecciosas & Parasitarias, Fac Med, Sao Paulo - Brazil
[7] Inst Infect Dis Infectol Emilio Ribas, Sao Paulo - Brazil
[8] Univ Sao Paulo, Sch Med, Heart Inst InCor, Sao Paulo - Brazil
Total Affiliations: 8
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 11, n. 1 OCT 28 2021.
Web of Science Citations: 0

We quantified the presence of SARS-CoV-2 RNA in the air of different hospital settings and the autopsy room of the largest medical centre in Sao Paulo, Brazil. Real-time reverse-transcription PCR was used to determine the presence of the envelope protein of SARS-CoV-2 and the nucleocapsid protein genes. The E-gene was detected in 5 out of 6 samples at the ICU-COVID-19 ward and in 5 out of 7 samples at the ward-COVID-19. Similarly, in the non-dedicated facilities, the E-gene was detected in 5 out of 6 samples collected in the ICU and 4 out of 7 samples in the ward. In the necropsy room, 6 out of 7 samples were positive for the E-gene. When both wards were compared, the non-COVID ward presented a significantly higher concentration of the E-gene than in the COVID-19 ward (p = 0.003). There was no significant difference in E-gene concentration between the ICU-COVID-19 and the ICU (p = 0.548). Likewise, there was no significant difference among E-gene concentrations found in the autopsy room versus the ICUs and wards (dedicated or not) (p = 0.245). Our results show the widespread presence of aerosol contamination in different hospital units. (AU)

FAPESP's process: 19/03397-5 - Identification and physical-chemical characterization of environmental microplastics in the atmosphere and in human lung tissues
Grantee:Luís Fernando Amato Lourenço
Support Opportunities: Scholarships in Brazil - Post-Doctorate