ffect of acute swimming exercise at different intensities but equal total load over metabolic and molecular responses in swimming rat

Author(s)

Maia Forte, Lucas Dantas ^[1] ; de Almeida Rodrigues, Natalia ^[1] ; Cordeiro, Andre Vitor ^[1] ; de Fante, Thais ^[2] ; de Paula Simino, Lais Angelica ^[2] ; Torsoni, Adriana de Souza ^[2] ; Torsoni, Marcio Alberto ^[2] ; Gobatto, Claudio Alexandre ^[1] ; Manchado-Gobatto, Fulvia Barros ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Appl Sport Physiol, UNICAMP, St 1300 Pedro Zaccaria, BR-13484350 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Sch Appl Sci, Lab Metab Disorders, St 1300 Pedro Zaccaria, BR-13484350 Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY; v. 43, n. 1, p. 35-44, MAR 2022.
• Web of Science Citations: 0

Abstract

Acute metabolic and molecular response to exercise may vary according to exercise's intensity and duration. However, there is a lack regarding specific tissue alterations after acute exercise with aerobic or anaerobic predominance. The present study investigated the effects of acute exercise performed at different intensities, but with equal total load on molecular and physiological responses in swimming rats. Sixty male rats were divided into a control group and five groups performing an acute bout of swimming exercise at different intensities (80, 90, 100, 110 and 120% of anaerobic threshold {[}AnT]). The exercise duration of each group was balanced so all groups performed at the same total load. Gene expression (HIF-1 alpha, PGC-1 alpha, MCT1 and MCT4 mRNA), blood biomarkers and tissue glycogen depletion were analyzed after the exercise session. ANOVA One-Way was used to indicate statistical mean differences considering 5% significance level. Blood lactate concentration was the only biomarker sensitive to acute exercise, with a significant increase in rats exercised above AnT intensities (p < 0.000). Glycogen stores of gluteus muscle were significantly reduced in all exercised animals in comparison to control group (p = 0.02). Hepatic tissue presented significant reduction in glycogen in animals exercised above AnT (p = 0.000, as well as reduced HIF-1 alpha mRNA and increased MCT1 mRNA, especially at the highest intensity (p = 0.002). Physiological parameters did not alter amongst groups for most tissues. Our results indicate the hepatic tissue alterations (glycogen stores and gene expressions) in response to different exercise intensities of exercise, even with the total load matched. (AU)

• FAPESP's process: 14/10336-9 - Effect of different training models with load control over physiological parameters, genic expressions and protein contents of HIF-1 alpha, PGC-1 alpha, MCT1 and MCT4: relationship with performance and spontaneous activity of swimming rats
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Regular Research Grants