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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

qsR toxin as a biotechnological tool for plant pathogen bacterial contro

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Author(s):
de Souza-Neto, Reinaldo Rodrigues [1, 2] ; Barbosa Carvalho, Isis Gabriela [2] ; Moreira Martins, Paula Maria [2] ; Picchi, Simone Cristina [2] ; Tomaz, Juarez Pires [3] ; Caserta, Raquel [2] ; Takita, Marco Aurelio [2] ; de Souza, Alessandra Alves [2]
Total Authors: 8
Affiliation:
[1] Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas, SP - Brazil
[2] Agron Inst IAC, Citrus Res Ctr, Cordeiropolis, SP - Brazil
[3] Rural Dev Inst Parana IAPAR EMATER, Londrina, Parana - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 12, n. 1 FEB 18 2022.
Web of Science Citations: 0
Abstract

Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control. (AU)

FAPESP's process: 17/13885-1 - Use of CRISPR/Cas9 in the editing of susceptibility genes of citrus sinensis to Xanthomonas citri subsp. Citri
Grantee:Reinaldo Rodrigues de Souza Neto
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/16142-0 - Translocation of quorum sensing molecules from transgenic rootstocks to sweet orange canopies: effect on resistance against phytopathogens
Grantee:Raquel Caserta Salviatto
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/10957-0 - Xylella fastidiosa-vector-host plant interaction and approaches for citrus variegated chlorosis and citrus canker control
Grantee:Alessandra Alves de Souza
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/15741-4 - Characterization of target genes MqsR toxin in Xylella fastidiosa: regulatory function and formation of persistent cells
Grantee:Isis Gabriela Barbosa Carvalho
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/50880-0 - INCT 2014: comparative and functional genomics and citrus-assisted breeding
Grantee:Marcos Antonio Machado
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/01273-9 - Toxin-antitoxin systems in Xanthomonas citri subsp citri: function and strategy for programmed cell death induction
Grantee:Paula Maria Moreira Martins
Support Opportunities: Scholarships in Brazil - Post-Doctoral