Short-term drought triggers defence mechanisms faster than ABA accumulation in the epiphytic bromeliad Acanthostachys strobilacea

Author(s)

Carvalho, Victoria ; Gaspar, Marilia ; Nievola, CatarinaC

• Document type: Journal article
• Source: Plant Physiology and Biochemistry; v. 160, p. 11-pg., 2021-01-15.

Abstract

Epiphytic bromeliads might experience drought after a few hours without water, which is especially critical during early life stages. Consequently, juvenile epiphytic bromeliads probably rely on short-term activation of drought tolerance strategies, although the biochemical processes involved are still poorly understood. In this study, we aimed to evaluate the short-term drought response of juvenile plants of the epiphytic bromeliad Acanthostachys strobilacea (Schult. & Schult. f.) Klotzsch. We hypothesized that short-term drought would induce the accumulation of abscisic acid (ABA) and secondary messengers such as reactive oxygen and nitrogen species (ROS and RNS, respectively) before the activation of defence mechanisms. Three-month-old plants were transferred from well-watered to dry substrates and stress markers were assessed at 0, 2, 5, 10, 24, 48, and 72 h. Drought caused a 27.3% decrease in relative water content compared to the well-watered control at 72 h. A nearly 5-fold increment in the ABA content occurred at 72 h of stress, which was about two days after the first detection of peaks in RNS levels and defence mechanisms activation. Indeed, ascorbate peroxidase (EC 1.11.1.11) activities and proline content increased after 10 h, whereas after 24 h a higher catalase (EC 1.11.1.6) activity and osmotic adjustment occurred. Oxidative stress markers and photochemical efficiency of photosystem II indicated no significant damage induced by drought. We concluded that defence mechanisms activation during early drought in juvenile A. strobilacea might be regulated initially by ABA-independent pathways and RNS, while ABA-induced responses are triggered at subsequent stages of stress. (AU)

• FAPESP's process: 17/50341-0 - Challenges for biodiversity conservation facing climate changes, pollution, land use and occupation (PDIp)
• Grantee: Luiz Mauro Barbosa
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 18/13529-3 - Involvement of nitro-oxidative metabolism, abscisic acid and aquaporins in short-term responses and recovery to drought in the epiphytic bromeliad Acanthostachys strobilacea (Schult. & Schult.f.) Klotzsch
• Grantee: Victória de Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate