Comprehensive expression analyses of the ABCG subfamily reveal SvABCG17 as a potential transporter of lignin monomers in the model C4 grass Setaria viridis

Author(s)

de Lima, Leydson Gabriel Alves ; Ferreira, Savio Siqueira ; Simoes, Marcella Siqueira ; Cunha, Lucas Xavier ; Fernie, Alisdair R. ; Cesarino, Igor

• Document type: Journal article
• Source: Journal of Plant Physiology; v. 280, p. 14-pg., 2022-12-14.

Abstract

Although several aspects of lignin metabolism have been extensively characterized, the mechanism(s) by which lignin monomers are transported across the plasma membrane remains largely unknown. Biochemical, proteomic, expression and co-expression analyses from several plant species support the involvement of active transporters, mainly those belonging to the ABC superfamily. Here, we report on the genome-wide characterization of the ABCG gene subfamily in the model C4 grass Setaria viridis and further identification of the members potentially involved in monolignol transport. A total of 48 genes encoding SvABCGs were found in the S. viridis genome, from which 21 SvABCGs were classified as full-size transporters and 27 as half-size transporters. Comprehensive analysis of the ABCG subfamily in S. viridis based on expression and co-expression analyses support a role for SvABCG17 in monolignol transport: (i) SvABCG17 is orthologous to AtABCG29, a monolignol transporter in Arabidopsis thaliana; (ii) SvABCG17 displays a similar expression profile to that of lignin biosynthetic genes in a set of different S. viridis tissues and along the elongating internode; (iii) SvABCG17 is highly coexpressed with lignin-related genes in a public transcriptomic database; (iv) SvABCG17displays particularly high expression in the top of the S. viridis elongating internode, a tissue undergoing active lignification; (v) SvABCG17 mRNA localization coincides with the histochemical pattern of lignin deposition; and (vi) the promoter of SvABCG17 is activated by secondary cell wall-associated transcription factors, especially by lignin-specific activators of the MYB family. Further studies might reveal further aspects of this potential monolignol transporter, including its real substrate specificity and whether it works redundantly with other ABC members during S. viridis lignification. (AU)

• FAPESP's process: 19/25587-0 - Going deep into the phenylpropanoid metabolism in C4 grasses using Setaria viridis as model species
• Grantee: Igor Cesarino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/14604-1 - The ABC of lignification: towards the identification of ABC transporters potentially involved in lignin deposition in Setaria viridis (L.) P.Beauv
• Grantee: Leydson Gabriel Alves de Lima
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/06917-1 - Transcriptomic analysis and co-expression network construction in model systems for identification of genes involved in secondary cell wall deposition and lignin metabolism in grasses
• Grantee: Sávio de Siqueira Ferreira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral