Potential of Pseudomonas and Trichoderma from the Brazilian Amazon as Biocontrol Agents against the Wheat Blast Disease

Author(s):Show less -

de Azambuja Pereira, Maikon Richer ; Moreira, Silvino Intra ; da Silva, Abimael Gomes ; Nunes, Tiago Calves ; Campos Vicentini, Samara Nunes ; da Silva, Davi Prata ; da Silveira, Patricia Ricardino ; Kiyama de Oliveira, Tamiris Yoshie ; Silva, Tatiane Carla ; dos Santos Botelho, Deila Magna ; Vilela Resende, Mario Lucio ; Ceresini, Paulo Cezar

• Document type: Journal article
• Source: AGRONOMY-BASEL; v. 12, n. 9, p. 17-pg., 2022-09-01.

Abstract

Blast is one of the most significant wheat diseases, causing high yield losses in susceptible varieties under favorable conditions in Latin America, Southeastern Asia and Eastern Africa. The disease is caused by the ascomycetous fungal pathogen Pyricularia oryzae Triticum lineage (PoTl). Chemical control with fungicides has been used as a management strategy; however, the effectiveness of the major classes of high-risk site-specific systemic fungicides has been reduced due to the widespread prevalence of resistance, especially in Brazil. Biological control is seen as a highly important and sustainable strategy to minimize the impact of yield losses associated with wheat blast in areas where fungicides are ineffective. In our study, we specifically aimed to determine the biological control potential of the three isolates of fluorescent Pseudomonas and three of Trichoderma as the antagonists of PoTl, both in in vitro and under greenhouse conditions. Additionally, we aimed to describe the ultrastructural interactions among the biocontrol agents and the pathogen in vitro by means of scanning electron microscopy (SEM). Fluorescent P. wayambapalatensis 'Amana' or Pseudomonas sp. nov. 'Yara', both from the P. putida group, and Trichoderma koningiopsis 'Cachara' significantly reduced PoTl in vitro mycelial growth and the blast disease severity on wheat plants. The SEM analyses revealed ultrastructural antagonistic mechanisms: biofilm formation, direct antagonism and mycoparasitism. Further research on the topic should include the development of stable formulations of the Pseudomonas- and Trichoderma-based biocontrol agents selected in our study for managing the wheat blast disease and the field tests of the biofungicide formulations obtained thereafter. (AU)

• FAPESP's process: 18/21197-0 - Novel real-time disease surveillance and fungicide resistance monitoring tools to foster a smart and sustainable crop protection platform in Brazil
• Grantee: Paulo Cezar Ceresini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/12509-1 - Novel real-time disease surveillance and fungicide resistance monitoring tools to foster a smart and sustainable crop protection platform in Brazil
• Grantee: Silvino Intra Moreira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/07611-9 - Development of a joint platform for disease epidemics monitoring and fungicide resistance detection to foster a smart program for management of wheat blast in Paraná and São Paulo states
• Grantee: Paulo Cezar Ceresini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/01675-5 - Pseudomonas and Trichoderma potential as biocontrol agents against wheat blast
• Grantee: Maikon Richer de Azambuja Pereira
• Support Opportunities: Scholarships in Brazil - Master