Transesterification of castor oil catalyzed by a fermented solid produced by Burkholderia contaminans using a bioreactor coupled with ultrasound irradiation

Author(s)

Coelho Moreira, Michelle Aparecida ; Alnoch, Robson Carlos ; de Lima Luz Junior, Luiz Fernando ; Baron, Alessandra Machado ; Mitchell, David Alexander ; Krieger, Nadia

• Document type: Journal article
• Source: Biotechnology and Applied Biochemistry; v. N/A, p. 11-pg., 2022-10-24.

Abstract

In this work, ultrasound was used to assist the ethanolysis of castor oil in a solvent-free system, catalyzed by a dry fermented solid containing the lipase from Burkholderia contaminans (BCFS). Reactions were done at 45 degrees C. The maximum conversion in Erlenmeyer flasks was 71% in 96 h, using a loading of 9% (mass of BCFS in relation to the mass of triacylglycerols in the castor oil) and a molar ratio of ethanol:oil of 6:1, with addition of ethanol in 12 steps. In a packed-bed reactor containing 12 g of BCFS, the conversions were 78% in 48 h, and 83% in 72 h with an ethanol to oil molar ratio of 3:1 and treatment with an ultrasound probe, with maximum power of 500 W, frequency of 20 kHz, and 75% of the maximum power. These results are promising given that, with an ultrasound assisted bioreactor, a higher conversion in a shorter time was achieved, with a lower ethanol to oil molar ratio than was the case in the Erlenmeyer flasks without ultrasound. (AU)

• FAPESP's process: 20/00081-4 - Development of strategies for immobilization and co-immobilization of enzymes involved in biomass degradation
• Grantee: Robson Carlos Alnoch
• Support Opportunities: Scholarships in Brazil - Post-Doctoral