Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria

Author(s)

de Menezes, Fabricia Farias ; Martim, Damaris Batistao ; Ling, Liu Yi ; Mulato, Aline Tieppo Nogueira ; Crespim, Elaine ; Oliveira, Juliana Velasco de Castro ; Driemeier, Carlos Eduardo ; de Giuseppe, Priscila Oliveira ; Rocha, George Jackson de Moraes

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 223, p. 8-pg., 2022-11-09.

Abstract

Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated. In this study, we evaluated the hydrothermal depolymerization of an beta-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 degrees C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v(-1)) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils). Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions. While the high-molecular-weight (heavy) bio-oil generated at 350 degrees C, 90 min, and 1:50 m.v(-1) failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species. The isolates Pseudomonas sp. LIM05 and Burkholderia sp. LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream. (AU)

• FAPESP's process: 19/22213-2 - Biological modification of lignin fragments in phenolic composition
• Grantee: George Jackson de Moraes Rocha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/06921-7 - Investigation of the metabolic pathways from Xanthomonas axonopodis pv. citri for the utilization of aromatic compounds derived from lignin
• Grantee: Priscila Oliveira de Giuseppe
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/00474-1 - Deciphering the role of bacterial volatile organic compounds in the promotion of maize (Zea mays L.) growth and health and in the modulation of its microbial community
• Grantee: Juliana Velasco de Castro Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/08590-8 - Identification of pathways for the catabolism of lignin-derived aromatic compounds in Xanthomonas axonopodis pv citri
• Grantee: Damaris Batistão Martim
• Support Opportunities: Scholarships in Brazil - Doctorate