| Full text | |
| Author(s): |
Camargo, Felipe N.
;
Matos, Sandro L.
;
Araujo, Layanne C. C.
;
Carvalho, Carla R. O.
;
Amaral, Andressa G.
;
Camporez, Joao Paulo
Total Authors: 6
|
| Document type: | Journal article |
| Source: | CURRENT ISSUES IN MOLECULAR BIOLOGY; v. 44, n. 10, p. 12-pg., 2022-10-01. |
| Abstract | |
One of the consequences of the Western lifestyle and high-fat diet is non-alcoholic fatty liver disease (NAFLD) and its aggressive form, non-alcoholic steatohepatitis (NASH), which can progress to cirrhosis and hepatocellular carcinoma (HCC) and is rapidly becoming the leading cause of end-stage liver disease or liver transplantation. Currently, rodent NASH models lack significant aspects of the full NASH spectrum, representing a major problem for NASH research. Therefore, this work aimed to characterize a fast rodent model with all characteristic features of NASH. Eight-week-old male ApoE KO mice were fed with Western diet (WD), high fatty diet (HFD) or normal chow (Chow) for 7 weeks. Whole-body fat was increased by similar to 2 times in WD mice and HFD mice and was associated with increased glucose intolerance, hepatic triglycerides, and plasma ALT and plasma AST compared with Chow mice. WD mice also showed increased galectin-3 expression compared with Chow or HFD mice and increased plasma cholesterol compared with Chow mice. WD and HFD displayed increased hepatic fibrosis and increased F4/80 expression. WD mice also displayed increased levels of plasma MCP-1. Hepatic inflammatory markers were evaluated, and WD mice showed increased levels of TNF-alpha, MCP-1, IL-6 and IFN-gamma. Taken together, these data demonstrated that the ApoE KO mouse fed with WD is a great model for NASH research, once it presents the fundamental parameters of the disease, including hepatic steatosis, fibrosis, inflammation, and metabolic syndrome. (AU) | |
| FAPESP's process: | 18/04956-5 - Impact of the estrogen receptor alpha on Non-Alcoholic Fatty Liver Disease and energetic metabolism of the liver |
| Grantee: | João Paulo Gabriel Camporez |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 19/18965-9 - Effect of increased estrogen receptor alpha expression by adeno-associated virus on hepatic metabolism |
| Grantee: | Felipe Nunes de Camargo |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 20/09094-1 - Impact of galectin-3 inhibition on hepatic insulin resistance and energy metabolism in Non-Alcoholic Steato-Hepatitis |
| Grantee: | Layanne Cabral da Cunha Araujo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |