Effects of rmBMP-7 on Osteoblastic Cells Grown on a Nanostructured Titanium Surface

Author(s)

Zuardi, Leonardo Raphael ; de Oliveira, Fabiola Singaretti ; Fernandes, Roger Rodrigo ; Oliveira Gomes, Maria Paula ; Spriano, Silvia ; Nanci, Antonio ; de Oliveira, Paulo Tambasco

• Document type: Journal article
• Source: BIOMIMETICS; v. 7, n. 3, p. 15-pg., 2022-09-01.

Abstract

This study evaluates the effects of the availability of exogenous BMP-7 on osteoblastic cells' differentiation on a nanotextured Ti surface obtained by chemical etching (Nano-Ti). The MC3T3-E1 and UMR-106 osteoblastic cell lines were cultured for 5 and 7 days, respectively, on a Nano-Ti surface and on a control surface (Control-Ti) in an osteogenic medium supplemented with either 40 or 200 ng/mL recombinant mouse (rm) BMP-7. The results showed that MC3T3-E1 cells exhibited distinct responsiveness when exposed to each of the two rmBMP-7 concentrations, irrespective of the surface. Even with 40 ng/mL rmBMP-7, important osteogenic effects were noticed for Control-Ti in terms of cell proliferation potential; Runx2, Osx, Alp, Bsp, Opn, and Smad1 mRNA expression; and in situ ALP activity. For Nano-Ti, the effects were limited to higher Alp, Bsp, and Opn mRNA expression and in situ ALP activity. On both surfaces, the osteogenic potential of UMR-106 cultures remained unaltered with 40 ng/mL rmBMP-7, but it was significantly reduced when the cultures were exposed to the 200 ng/mL concentration. The availability of rmBMP-7 to pre-osteoblastic cells at the concentrations used alters the expression profile of osteoblast markers, indicative of the acquisition of a more advanced stage of osteoblastic differentiation. This occurs less pronouncedly on the nanotextured Ti and without reflecting in higher mineralized matrix production by differentiated osteoblasts on both surfaces. (AU)

• FAPESP's process: 16/50298-4 - Natural molecules on the surface of bioactive materials for modulating the host response to implants (NAT4MORE)
• Grantee: Paulo Tambasco de Oliveira
• Support Opportunities: Regular Research Grants