| Full text | |
| Author(s): |
Koltun, Alessandra
;
Maniero, Rodolfo A. A.
;
Vitti, Marielle
;
de Setta, Nathalia
;
Giehl, Ricardo F. H.
;
Lima, Joni E. E.
;
Figueira, Antonio
Total Authors: 7
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PLANT SCIENCE; v. 13, p. 16-pg., 2022-11-18. |
| Abstract | |
AMMONIUM TRANSPORTER/METHYLAMMONIUM PERMEASE/RHESUS (AMT) family members transport ammonium across membranes in all life domains. Plant AMTs can be categorized into AMT1 and AMT2 subfamilies. Functional studies of AMTs, particularly AMT1-type, have been conducted using model plants but little is known about the function of AMTs from crops. Sugarcane (Saccharum spp.) is a major bioenergy crop that requires heavy nitrogen fertilization but depends on a low carbon-footprint for competitive sustainability. Here, we identified and functionally characterized sugarcane ScAMT2;1 by complementing ammonium uptake-defective mutants of Saccharomyces cerevisiae and Arabidopsis thaliana. Reporter gene driven by the ScAMT2;1 promoter in A. thaliana revealed preferential expression in the shoot vasculature and root endodermis/pericycle according to nitrogen availability and source. Arabidopsis quadruple mutant plants expressing ScAMT2;1 driven by the CaMV35S promoter or by a sugarcane endogenous promoter produced significantly more biomass than mutant plants when grown in NH4+ and showed more N-15-ammonium uptake by roots and nitrogen translocation to shoots. In A. thaliana, ScAMT2;1 displayed a K-m of 90.17 mu M and V-max of 338.99 mu moles h(-1) g(-1) root DW. Altogether, our results suggest that ScAMT2;1 is a functional high-affinity ammonium transporter that might contribute to ammonium uptake and presumably to root-to-shoot translocation under high NH4+ conditions. (AU) | |
| FAPESP's process: | 10/11313-1 - Identification, physiological characterization and gene expression profilling of sugarcane (Saccharum spp.) cultivars contrasting for nitrogen use efficiency |
| Grantee: | Joni Esrom Lima |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/15989-8 - Physiological, biochemical and molecular characterizaiton of nitrate transport during n uptake and remobilization in sugarcane (Saccharum spp.) |
| Grantee: | Antonio Vargas de Oliveira Figueira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/00460-2 - Functional characterization of gene encoding ammonium transporter ScAMT3.3 in sugarcane (Saccharum spp.) |
| Grantee: | Rodolfo Augusto Maniero |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/14669-8 - Functional characterization of sugarcane (Saccharum spp.) genes encoding ammonium transporters |
| Grantee: | Antonio Vargas de Oliveira Figueira |
| Support Opportunities: | Regular Research Grants |