Viability of ex-vivo myography as a diagnostic tool for rectus abdominis muscle electrical activity collected at Cesarean section within a diamater cohort study

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Reyes, David R. A. ; Barbosa, Angelica M. P. ; Juliana, Floriano F. ; Sofia, Quiroz B. C., V ; Costa, Sarah M. B. ; Hallur, Raghavendra L. S. ; Enriquez, Eusebio M. A. ; Oliveira, Rafael G. ; Rossignolli, Patricia de Souza ; Pedroni, Cristiane Rodrigues ; Alves, Fernanda C. B. ; Garcia, Gabriela A. ; Abbade, Joelcio F. ; Carvalho, Carolina N. F. ; Sobrevia, Luis ; Rudge, Marilza V. C. ; Calderon, Iracema I. M. P. ; Diamater Study Grp

• Document type: Journal article
• Source: BIOMEDICAL ENGINEERING ONLINE; v. 21, n. 1, p. 12-pg., 2022-10-14.

Abstract

Background Ex-vivo myography enables the assessment of muscle electrical activity response. This study explored the viability of determining the physiological responses in muscles without tendon, as rectus abdominis muscle (RAM), through ex-vivo myography to assess its potential as a diagnostic tool. Results All tested RAM samples (five different samples) show patterns of electrical activity. A positive response was observed in 100% of the programmed stimulation. RAM 3 showed greater weight (0.47 g), length (1.66 cm), and width (0.77 cm) compared to RAM 1, RAM 2, RAM 4 and RAM 5 with more sustained electrical activity over time, a higher percentage of fatigue was analyzed at half the time of the electrical activity. The order of electrical activity (Mn) was RAM 3 > RAM 5 > RAM 1 > RAM 4 > RAM 2. No electrical activity was recorded in the Sham group. Conclusions This study shows that it is feasible to assess the physiological responses of striated muscle without tendon as RAM, obtained at C-section, under ex vivo myography. These results could be recorded, properly analyzed, and demonstrated its potential as a diagnostic tool for rectus abdominis muscle electrical activity. (AU)

• FAPESP's process: 16/01743-5 - The new gestational triad: hyperglycemia, urinary incontinence (UI) and biomolecular profile as a long-term predictor for UI: a prospective cohort study: translational research with biodevice with stem cells for muscle regeneration in diabetic rats
• Grantee: Marilza Vieira Cunha Rudge
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/10661-8 - Gestational triad hyperglycemia cohort, urinary incontinence and clinical profile, molecular and omic of hyperglycemic myopathy in the prediction of incontinence and muscle dysfunction and translational research with biodevic for muscle regeneration in ra
• Grantee: David Rafael Abreu Reyes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral