| Full text | |
| Author(s): |
Dias Rosa, Higor Vinicius
;
Leonardo, Diego Antonio
;
Brognara, Gabriel
;
Brandao-Neto, Jose
;
Pereira, Humberto D'Muniz
;
Ulian Araujo, Ana Paula
;
Garratt, Richard Charles
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Journal of Molecular Biology; v. 432, n. 21, p. 18-pg., 2020-10-02. |
| Abstract | |
The assembly of a septin filament requires that homologous monomers must distinguish between one another in establishing appropriate interfaces with their neighbors. To understand this phenomenon at the molecular level, we present the first four crystal structures of heterodimeric septin complexes. We describe in detail the two distinct types of G-interface present within the octameric particles, which must polymerize to form filaments. These are formed between SEPT2 and SEPT6 and between SEPT7 and SEPT3, and their description permits an understanding of the structural basis for the selectivity necessary for correct filament assembly. By replacing SEPT6 by SEPT8 or SEPT11, it is possible to rationalize Kinoshita's postulate, which predicts the exchangeability of septins from within a subgroup. Switches I and II, which in classical small GTPases provide a mechanism for nucleotide-dependent conformational change, have been repurposed in septins to play a fundamental role in molecular recognition. Specifically, it is switch I which holds the key to discriminating between the two different G-interfaces. Moreover, residues which are characteristic for a given subgroup play subtle, but pivotal, roles in guaranteeing that the correct interfaces are formed. Crown Copyright (C) 2020 Published by Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 14/15546-1 - Septins: comparative studies and the correlation between structure and function |
| Grantee: | Richard Charles Garratt |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/04658-9 - Structural determinants for specific interaction of interfaces G and NC of septins: validating the substitution rules in the assembly of the filament |
| Grantee: | Diego Antonio Leonardo Cabrejos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |