High-fat diet decreases H3K27ac in mice adipose-derived stromal cells

Author(s)

da Silva, Viviane S. ; Simao, Jussara J. ; Plata, Victor ; de Sousa, Andressa Franca ; da Cunha de Sa, Roberta D. C. ; Machado, Cleiton Fagundes ; Stumpp, Taiza ; Alonso-Vale, Maria Isabel C. ; Armelin-Correa, Lucia

• Document type: Journal article
• Source: OBESITY; v. 30, n. 10, p. 10-pg., 2022-09-05.

Abstract

Objective The study goal was to analyze the effects of a high-fat diet (HFD) on the histone 3 lysine 27 (H3K27) posttranscriptional modifications and the expression of histone-modifying enzymes in adipose-derived stromal cells (ASCs) from white adipose tissue (WAT). Methods Male C57BL/6J mice received control or HFD for 12 weeks. The ASCs were isolated from subcutaneous and visceral (epididymal) WAT, cultivated, and evaluated for expression of H3K27 trimethylation (H3K27me3) and H3K27 acetylation (H3K27ac) by Western blot. The transcription of histone-modifying enzymes was analyzed by real-time polymerase chain reaction. Results When compared with control, HFD ASCs showed a decrease in H3K27ac enrichment in subcutaneous and visceral WAT and ATP-citrate lyase expression in subcutaneous WAT. Curiously, the expression of CREB-binding protein was increased in visceral ASCs from HFD-fed mice. Conclusions These results show that an HFD significantly reduces acetylation of H3K27 in ASCs and the expression of ATP-citrate lyase in subcutaneous ASCs, suggesting that, in this fat depot, the H3K27ac reduction could be partly due to lower acetyl-coenzyme A availability. H3K27ac is an epigenetic mark responsible for increasing the transcription rate and its reduction can have an important impact on ASC proliferation and differentiation potential. (AU)

• FAPESP's process: 19/13618-9 - Modulation of Ezh2 expression, and other H3K27 modifiers, by NF-kB transcription factors in adipose cells
• Grantee: Maria Isabel Cardoso Alonso-Vale
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/26240-4 - Signaling pathways affected by fish oil in Adipose tissue-Derived Stem Cells (AdSCs) and adipocytes: correlation with metabolic, endocrine and adipogenic changes that impact its anti-Hypertrophic Obesity properties
• Grantee: Jussara de Jesus Simão
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/05485-6 - Effects of high fat diet and supplementation with omega-7 and omega-3 fatty acids on the browning process, proliferation and differentiation of mesenchymal cells contained in the stromal cell fraction from adipose tissue
• Grantee: Maria Isabel Cardoso Alonso-Vale
• Support Opportunities: Regular Research Grants