Taxonomy and 18S rDNA-based phylogeny of Henneguya multiradiatus n. sp. (Cnidaria: Myxobolidae) a parasite of Brochis multiradiatus from Peruvian Amazon

Author(s)

Mathews, Patrick D. ; Mertins, Omar ; Espinoza, Luis L. ; Milanin, Tiago ; Alama-Bermejo, Gema ; Audebert, Fabienne ; Morandini, Andre C.

• Document type: Journal article
• Source: Microbial Pathogenesis; v. 147, p. 7-pg., 2020-10-01.

Abstract

A new myxozoan species belonging to the genus Henneguya was isolated from the serous membrane of the visceral cavity of the hognosed catfish Brochis multiradiatus from Peruvian Amazon. Whitish plasmodia, macroscopically visible, were found in four of the thirty examined fishes. Mature myxospores were ellipsoidal in shape in frontal view and had a total length of 44.5 +/- 0.6 mu m (43.9-45.1), spore body measured 18.7 +/- 0.9 mu m (16.8-19.6) in length, 7.1 +/- 0.2 mu m (6.6-7.4) in width and 5.5 +/- 0.3 mu m (4.9-5.6) in thickness. The two polar capsules were elongated and equal in size, measuring 9.1 +/- 0.1 mu m (8.8-9.4) in length and 1.7 +/- 0.1 mu m (1.6-1.8) in width, occupying half of the myxospore body. Polar tubules coiled in 10-11 turns perpendicular to the long axis of the polar capsule. The caudal appendage was not bifurcated and measured 25.8 +/- 0.6 mu m (24.7-26.5) in length. The sequencing of the 18S rDNA gene resulted in 1400 bp and this sequence did not match any of the myxozoans available in GenBank. Phylogenetic analysis placed the new species in a well-supported subclade of Henneguya spp. infecting callichthyid fishes, with Henneguya loretoensis being the closest species. This study is the first description of a myxozoan species, Henneguya multiradiatus n. sp. from a fish of the genus Brochis. (AU)

• FAPESP's process: 15/18807-3 - Detection of myxosporean in oligochaetes from fish farms of hybrid pintado (Pseudoplatystoma corruscans x Pseudoplatystoma reticulatum) in Mato Grosso do Sul state, Brazil
• Grantee: Tiago Milanin
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/13147-6 - Studies of the differential expressed immune genes in organs of ornamental fish in response to myxosporeans infection
• Grantee: Patrick Mathews Delgado
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 18/20482-3 - Diversity of microscopic cnidarians from the Amazon Basin with emphasis on phylogeny, systematic and interaction with their hosts
• Grantee: Patrick Mathews Delgado
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/21007-9 - Recognizing the diversity of jellyfishes (Medusozoa, Rhopaliophora)
• Grantee: André Carrara Morandini
• Support Opportunities: Regular Research Grants