TIP1;1 expression could modulate the recovery of stomatal opening during rehydration in Sorghum bicolor

Author(s)

Schley, Thayssa Rabelo ; Franco, Danilo Miralha ; Araujo Junior, Joao Pessoa ; Maia, Ivan de Godoy ; Habermann, Gustavo ; Rolim de Almeida, Luiz Fernando

• Document type: Journal article
• Source: ENVIRONMENTAL AND EXPERIMENTAL BOTANY; v. 200, p. 11-pg., 2022-05-21.

Abstract

Stomatal conductance (g(s)) takes longer to recover after drought when compared to leaf water potential or leaf water content (RWC). Aquaporins, known for transporting water through membranes, could modulate g(s) recovery. Using Sorghum bicolor, we tested whether members of the plasma membrane (PIP) and tonoplast (TIP) intrinsic aquaporin subfamilies could influence g(s) after a 20-day drought. Potted plants under soil field capacity (control), severe water deficit (SWD; 30% (m/m) soil water content), and progressive severe water deficit (PSWD; water withheld until maximum stress) were used. Soil (Psi(soil)), predawn (Psi(PD)), and midday (Psi(MD)) leaf water potentials, photochemical, and gas exchange parameters were measured during drought and 12 days of recovery. After 17 days of drought, g(s) of SWD and PSWD was 4-times lower than control, and it took 6 days to recover. Psi(PD), Psi(MD), and RWC, however, recovered after 24 h. SbPIP1;2, SbPIP2;5, and SbTIP1;1 were all downregulated on the day of maximum stress, but only SbTIP1;1 showed consistent alleviation of its downregulation as g(s) recovered. SbTIP1;1 expression was positively correlated with g(s), leaf transpiration, and hydraulic conductivity (K-L), but only in SWD plants. The slow g(s) recovery seems to be associated with the alleviation of SbTIP1;1 down-regulation as rehydration progresses. (AU)

• FAPESP's process: 10/15585-6 - Effect of drought on the allelopathic potential of copaiba (Copaifera langsdorffii Desf.)
• Grantee: Luiz Fernando Rolim de Almeida
• Support Opportunities: Regular Research Grants