| Full text | |
| Author(s): |
Roman-Campos, Danilo
;
Sales-Junior, Policarpo
;
Costa, Alexandre D.
;
Souza, Diego Santos
;
Santos-Miranda, Artur
;
Joviano-Santos, Julliane, V
;
Ropert, Catherine
;
Cruz, Jader S.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | MICROORGANISMS; v. 10, n. 2, p. 7-pg., 2022-02-01. |
| Abstract | |
Chagas disease (CD) is caused by the parasitic protozoan T. cruzi. The progression of CD in similar to 30% of patients results in Chagasic Cardiomyopathy (CCM). Currently, it is known that the inflammatory system plays a significant role in the CCM. Interferon-gamma (IFN-gamma) is the major cytokine involved in parasitemia control but has also been linked to CCM. The L-type calcium current (I-Ca,I-L) is crucial in the excitation/contraction coupling in cardiomyocytes. Thus, we compared I-Ca,I-L and the mechanical properties of cardiomyocytes isolated from infected wild type (WT) and IFN-gamma((-/-)) mice in the first stage of T. cruzi infection. Using the patch clamp technique, we demonstrated that the infection attenuated I-Ca,I-L in isolated cardiomyocytes from the right and left ventricles of WT mice at 15 days post-infection (dpi), which was not observed in the IFN-gamma((-/-)) cardiomyocytes. However, I-Ca,I-L was attenuated between 26 and 30 dpi in both experimental groups. Interestingly, the same profile was observed in the context of the mechanical properties of isolated cardiomyocytes from both experimental groups. Simultaneously, we tracked the mortality and MCP-1, TNF-alpha, IL-12, IL-6, and IL-10 serum levels in the infected groups. Importantly, the IFN-gamma((-/-)) and WT mice presented similar parasitemia and serum inflammatory markers at 10 dpi, indicating that the modifications in the cardiomyocyte functions observed at 15 dpi were directly associated with IFN-gamma((-/-)) deficiency. Thus, we showed that IFN-gamma plays a crucial role in the electromechanical remodeling of cardiomyocytes during experimental T. cruzi infection in mice. (AU) | |
| FAPESP's process: | 19/18918-0 - Role of nitric oxide in cardiac arrhythmias during Hypothyroidism |
| Grantee: | Diego Santos de Souza |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/22830-9 - Involvement of the Ca2+/ Calmodulin/CAMKII signaling axis in the electrical and contractile dysfunctions of the heart in the chronic phase of Chagas Disease |
| Grantee: | Artur Santos Miranda |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 19/21304-4 - Arrhythmogenic mechanisms in right heart diseases |
| Grantee: | Danilo Roman Campos |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/20777-3 - Genetic variants of the Nav 1.5 sodium channel and its therapeutic implications. |
| Grantee: | Julliane Vasconcelos Joviano dos Santos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |