| Full text | |
| Author(s): |
Durski, Mariana
;
Ravel, Jacques
;
Spautz, Ana C. P. G.
;
Carvalho, Newton S.
;
Silva, Marcia G.
;
Marconi, Camila
Total Authors: 6
|
| Document type: | Journal article |
| Source: | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 104, n. 1, p. 5-pg., 2022-06-30. |
| Abstract | |
Objective: To compare the diagnosis of bacterial vaginosis (BV) by Nugent scoring criteria (Nugent-BV) and the diagnosis of BV and/or aerobic vaginitis (AV) using Donders criteria (Donders-BV/AV) for identifying Molecular-BV detected by bacterial 16s rRNA profiling. Methods: We enrolled 512 women of reproductive age in Brazil with data available on Nugent and Donders microscopic analysis and 16S rRNA sequencing. We constructed receiver operating characteristic (ROC) curves of Nugent-BV and Donders-BV/AV and calculated their area under the curves (AUCs) and 95% confidence interval (CI) for matching Molecular-BV. Results: A total of 155 (28.7%) participants were positive for Nugent-BV. Donders-BV and -AV were detected in 90 (17.6%) and 75 (14.6%) participants, respectively, while 28 (5.5%) had concurrent Donders-BV and -AV. Molecular-BV was identified in 139 (27.1%) participants. Analysis of ROC curves showed that diagnosis of Nugent-BV more accurately aligned with presence of Molecular-BV (AUC: 0.88, 95% CI: 0.84-0.91) when compared to Donders-AV/BV (AUC: 0.84; CI: 0.80-0.87) (P = 0.005). Conclusion: The use of Nugent-BV is more representative of Molecular-BV than Donders-AV/BV. (c) 2022 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 12/10403-2 - Characterization of the vaginal microbiome of Brazilian women of reproductive age |
| Grantee: | Camila Marconi |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/16800-3 - Characterization of the vaginal microbiome of Brazilian women in reproductive age |
| Grantee: | Márcia Guimarães da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/22463-5 - Deep proteomic analysis of cervicovaginal fluid in bacterial vaginosis: association with bacterial composition and response to metronidazole treatment |
| Grantee: | Camila Marconi |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |