| Full text | |
| Author(s): |
Seiblitz, I. G. L.
;
Vaga, C. F.
;
Capel, K. C. C.
;
Cairns, S. D.
;
Stolarski, J.
;
Quattrini, A. M.
;
Kitahara, M., V
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Molecular Phylogenetics and Evolution; v. 175, p. 13-pg., 2022-07-19. |
| Abstract | |
Molecularly, the family Caryophylliidae is polyphyletic and different sets of genetic data converge towards a consensus that a taxonomic review of this family is necessary. Overall, the order of genes in the mitochondrial genome (mitogenome) together with DNA sequences have been used to successfully untangle evolutionary relationships in several groups of organisms. Published mitogenomes of two caryophylliid genera (Desmophyllum and Solenosmilia) present a transposition of the gene block containing cob, nad2, and nad6, which is located between nad5 5' exon and trnW, while that of Polycyathus chaishanensis presents the same gene order as the majority of scleractinian corals. In molecular-based evolutionary reconstructions, caryophylliids that have the mitochondrial gene rearrangement were recovered as a monophyletic lineage ("true" caryophylliids), while members of the genus Polycyathus were placed in a different position. In this study, additional mitogenomes of this family were assembled and included in evolutionary reconstructions of Scleractinia in order to improve our understanding on whether the mitogenome gene rearrangement is limited to and, therefore, could be a synapomorphy of the actual members of Caryophylliidae. Specimens of Caryophyllia scobinosa, Premocyathus sp., Heterocyathus sulcatus, and Trochocyathus caryophylloides, as well as Desmophyllum pertusum and Solenosmilia variabilis from the Southwest Atlantic were sequenced using Illumina platforms. Then, mitochondrial genomes were assembled and annotated, and nuclear datasets were recovered in-silico from assembled contigs using a previously published set of baits. Evolutionary reconstructions were performed using mitochondrial and nuclear datasets and based on Maximum Likelihood and Bayesian Inference. Obtained mitogenomes are circular and range between 15,816 and 18,225 bp in size and from 30.76% to 36.63% in GC content. The gene rearrangement is only seen in C. scobinosa, D. pertusum, Premocyathus sp., and S. variabilis, which were recovered as a monophyletic Glade in both mitochondrial and nuclear phylogenies. On the other hand, the "caryophylliids" with the canonical mitogenome gene order were not recovered within this Glade. Differences in features of the skeleton of "true" caryophylliids in comparison to traditional members of the family were observed and offer further support that the gene rearrangement might be seen as a synapomorphy of family Caryophylliidae. (AU) | |
| FAPESP's process: | 17/11615-7 - Phylogenomics of the order Scleractinia (Cnidaria, Anthozoa): relationships between evolution and climate change |
| Grantee: | Isabela Galvão de Lossio e Seiblitz |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/01332-0 - Phylogenomics of the order Scleractinia (Cnidaria, Anthozoa): relationships between evolution and climate change |
| Grantee: | Marcelo Visentini Kitahara |
| Support Opportunities: | Research Program on Global Climate Change - Young Investigators |
| FAPESP's process: | 18/19661-0 - Mitogenomes of scleractinian corals in Singapore |
| Grantee: | Isabela Galvão de Lossio e Seiblitz |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |
| FAPESP's process: | 17/50229-5 - Deep sea corals in the South Atlantic: new insights from an interdisciplinary study |
| Grantee: | Marcelo Visentini Kitahara |
| Support Opportunities: | Regular Research Grants |