Simultaneous UHPLC-MS Quantification of Catechins and Untargeted Metabolomic Profiling for Proof-of-Concept Authenticity Determination of Maytenus ssp. Samples

Author(s)

Duarte, Rodolfo Santos ; Miranda Antunes, Elisa Ribeiro ; Helena Frankland Sawaya, Alexandra Christine

• Document type: Journal article
• Source: Molecules; v. 27, n. 17, p. 9-pg., 2022-09-01.

Abstract

Due to the widespread use of Maytenus ilicifolia leaves in the treatment of gastric ulcers, herbal medicines derived from such species are distributed by the national health system in Brazil. A related species, Maytenus aquifolium, is also used for the same disorders, and both are popularly known as Espinheira-santa. Due to their popular use, the quality and efficiency of the herbal medicines derived from these species is an important public health issue. The purpose of this study was to develop and test an analytical method that could quantify the content of catechin and epicatechin in dry Maytenus spp. leaves and simultaneously obtain their chemical profile to determine authenticity of the leaf samples. Ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) was used to quantify these isomers, in the selected ion monitoring (SIM) mode, while simultaneously analyzing the extract in full-scan mode. This approach was successfully applied to the analysis of commercial and authentic samples of Maytenus spp. Fewer than half the samples presented the minimum epicatechin content of 2.8 mg per g of dry leaf mass, as specified in the 6th Brazilian Pharmacopoeia (2019) for M. ilicifolia. Furthermore, by using untargeted metabolomics, it was observed that the chemical profile of most the samples was not compatible with M. ilicifolia leaves, indicating the need for stricter quality control of this material. The method described herein could be used for this control; moreover, its concept could be adapted and used for an ample variety of medicinal plant products. (AU)

• FAPESP's process: 17/00155-5 - Seasonal variation of the chemical composition and antioxidant activity extracts of leaves of Maytenus ilicifolia Mart. ex Reiss, Maytenus aquifolium Mart (Celastraceae) and their hybrids
• Grantee: Elisa Ribeiro Miranda Antunes
• Support Opportunities: Scholarships in Brazil - Master