Polymorphisms in risk genes of type 2 diabetes mellitus could be also markers of susceptibility to periodontitis

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Silva, Arles Naisa Amaral ; Nicchio, Ingra Gagno ; da Silva, Barbara Roque ; Martelli, Mauricio Gandini Giani ; Hidalgo, Marco A. Rimachi ; Nepomuceno, Rafael ; Theodoro, Leticia H. ; Cirelli, Joni A. ; Orrico, Silvana R. P. ; Cirelli, Thamiris ; Barros, Silvana P. ; Scarel-Caminaga, Raquel M.

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 143, p. 12-pg., 2022-09-01.

Abstract

Objectives: This study aimed to investigate polymorphisms in genes considered molecular biomarkers of type 2 diabetes mellitus (T2DM) to assess whether they are associated with periodontitis, and relating them to the periodontal status, glycemic and lipid profile of the subjects. Design: We investigated individuals who underwent complete periodontal examination and biochemical evalu-ation. We categorized them into three groups: (i) periodontitis with T2DM (Periodontitis+T2DM group, n = 206); (ii) periodontitis without T2DM (Periodontitis group, n = 346); and (iii) healthy individuals without Periodontitis or T2DM (Healthy group, n = 345). We investigated three single nucleotide polymorphisms (SNPs) for AGER, RBMS1 and VEGFA genes. We applied multivariate logistic and multiple linear regression models for all groups and stratified the subjects by sex and smoking habits.Results: Compared with RBMS1-rs7593730-CC+CT genotype carriers, RBMS1-rs7593730-TT carriers were more susceptible to periodontitis [odds ratio (OR) = 2.29; 95% confidence interval (CI) = 1.04-5.01; P-value = 0.033]. Among AGER-rs184003-CC carriers, never smokers had reduced risks of periodontitis and Periodontitis+T2DM than ever smokers. For either RBMS1-rs7593730-CC or VEGFA-rs9472138-CC carriers, never smokers had less susceptibility to develop periodontitis than ever smokers. Compared with AGER-rs184003-CC carriers, AGER- rs184003-AA carriers presented fewer remaining teeth. VEGFA-rs9472138-TT carriers showed a lower per-centage of sites with characteristics of active periodontal disease (bleeding on pocket probing and interproximal clinical attachment level) compared with VEGFA-rs9472138-CC carriers.Conclusions: In the studied population, AGER rs184003, RBMS1 rs7593730, and VEGFA rs9472138, which are considered genetic markers for T2DM, were associated with periodontitis without T2DM or periodontitis together with T2DM. (AU)

• FAPESP's process: 16/18313-3 - Investigation of genetic variations susceptibility to chronic periodontitis by OpenArray®: Biostatistics, Bioinformatics and Functional Analysis
• Grantee: Rafael Nepomuceno Oliveira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/08070-6 - Investigation of genetic susceptibility to chronic periodontitis associated with Type 2 Diabetes mellitus
• Grantee: Thamiris Cirelli
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 14/13295-1 - Investigation of the association of genetic variation regarding susceptibility to chronic periodontal disease by OpenArray
• Grantee: Rafael Nepomuceno Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate