Exploring chemical diversity in Glycine max cultivars: A multivariate approach in the search for bioactive compounds against Spodoptera cosmioides

Author(s)

Aguiar, Maria Clara Santana ; de Freitas, Marcelo Mueller ; de Freitas, Carlos Alessandro ; Boica Junior, Arlindo Leal ; Carneiro, Renato Lajarim ; da Silva, Maria Fatima das Gracas Fernandes ; Fernandes, Joao Batista ; Forim, Moacir Rossi

• Document type: Journal article
• Source: FRONTIERS IN PLANT SCIENCE; v. 13, p. 18-pg., 2022-09-02.

Abstract

Soybean crop is regulated by abiotic and biotic stresses with great potential in reducing grain yield and quality. The selection of resistant cultivars is a promising approach for mitigating these damages. We evaluated the chemical profile of Glycine max leaves from different cultivars in order to explore their defense mechanisms against Spodoptera cosmioides caterpillars. We optimized solid-liquid extraction techniques using ultrasound bath and static headspace extraction. Additionally, we developed an approach based on liquid and gas chromatography for analyzing the chemical profile of G. max cultivars. The principal component analysis allowed the classification of transgenic cultivars, which are classified as susceptible to S. cosmioides, from those obtained by genetic improvement and resistant to the insect. Differences were observed in the abundance of phenolic glycosides, lipids, aldehydes, and alcohols. More specifically, S. cosmioides resistant cultivars presented molecules related to the jasmonic and salicylic acid pathways. Such data can contribute to a molecular understanding of phenotypic diversity in soybean cultivars, from plant quality to resistance mechanisms and adaptation, to environmental stress and herbivory. (AU)

• FAPESP's process: 18/00340-0 - Evaluation of environmental factors on the metabolic profile of Gossypium hirsutum L., resistant (FMT-701) and susceptible (DeltaOpal), and its relation with detoxification mechanisms promoted by microorganisms associated to insect pests
• Grantee: Moacir Rossi Forim
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/21201-8 - Novel delivery of phytochemicals for sustainable crop protection
• Grantee: Maria Fátima das Graças Fernandes da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/50918-7 - INCT 2014: for the Biorational Control of Pest-Insect and Phytopathogens
• Grantee: Maria Fátima das Graças Fernandes da Silva
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/11878-3 - Search for bioactive molecules to pest insect control through comparative metabolomic analysis among genotypes of commercial interest
• Grantee: Moacir Rossi Forim
• Support Opportunities: Regular Research Grants