| Full text | |
| Author(s): Show less - |
Alves, Fernanda Cristina Bergamo
;
de Oliveira, Rafael Guilen
;
Reyes, David Rafael Abreu
;
Garcia, Gabriela Azevedo
;
Floriano, Juliana Ferreira
;
Shetty, Raghavendra Hallur Lakshmana
;
Mareco, Edson Assuncao
;
Dal-Pai-Silva, Maeli
;
Payao, Spencer Luiz Marques
;
Souza, Fatima Pereira de
;
Witkin, Steven S.
;
Sobrevia, Luis
;
Barbosa, Angelica Mercia Pascon
;
Rudge, Marilza Vieira Cunha
;
Diamater Study Grp
Total Authors: 15
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 21, p. 20-pg., 2022-11-01. |
| Abstract | |
Gestational diabetes mellitus (GDM) is recognized as a "window of opportunity" for the future prediction of such complications as type 2 diabetes mellitus and pelvic floor muscle disorders, including urinary incontinence and genitourinary dysfunction. Translational studies have reported that pelvic floor muscle disorders are due to a GDM-induced-myopathy (GDiM) of the pelvic floor muscle and rectus abdominis muscle (RAM). We now describe the transcriptome profiling of the RAM obtained by Cesarean section from GDM and non-GDM women with and without pregnancy-specific urinary incontinence (PSUI). We identified 650 genes in total, and the differentially expressed genes were defined by comparing three control groups to the GDM with PSUI group (GDiM). Enrichment analysis showed that GDM with PSUI was associated with decreased gene expression related to muscle structure and muscle protein synthesis, the reduced ability of muscle fibers to ameliorate muscle damage, and the altered the maintenance and generation of energy through glycogenesis. Potential genetic muscle biomarkers were validated by RT-PCR, and their relationship to the pathophysiology of the disease was verified. These findings help elucidate the molecular mechanisms of GDiM and will promote the development of innovative interventions to prevent and treat complications such as post-GDM urinary incontinence. (AU) | |
| FAPESP's process: | 18/10661-8 - Gestational triad hyperglycemia cohort, urinary incontinence and clinical profile, molecular and omic of hyperglycemic myopathy in the prediction of incontinence and muscle dysfunction and translational research with biodevic for muscle regeneration in ra |
| Grantee: | David Rafael Abreu Reyes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 19/05710-2 - Prospective cohort of the omic biomarkers of the abdominal rectus muscle of pregnant women with hyperglycemia and pregnancy-specific urinary incontinence as predictors of urinary incontinence 6-12 months postpartum. |
| Grantee: | Fernanda Cristina Bérgamo Alves |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/01743-5 - The new gestational triad: hyperglycemia, urinary incontinence (UI) and biomolecular profile as a long-term predictor for UI: a prospective cohort study: translational research with biodevice with stem cells for muscle regeneration in diabetic rats |
| Grantee: | Marilza Vieira Cunha Rudge |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/21783-4 - Gestational triad cohort: hyperglycemia, urinary incontinence and clinical, molecular and omic profile of hyperglycemic myopathy in predicting incontinence and muscle dysfunction and translational research with biodevice for muscle regeneration in rats |
| Grantee: | Juliana Ferreira Floriano |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/26305-6 - Skeletal muscle organoid of pregnant women with Gestational Hyperglycemia and pregnancy-specific Urinary Incontinence as funcional 3D model to research biomarkers of Gestational Hyperglycemic Myopathy |
| Grantee: | Rafael Guilen de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/02979-8 - Prospective cohort of biochemical, protein and immunohistochemical markers of muscle fiber and extracellular matrix of the rectus abdominis muscle of Hyperglycemia and gestational-specific urinary incontinence as predictor of UI 6-12 months post-cesarean |
| Grantee: | Raghavendra Hallur Lakshmana Shetty |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |