| Full text | |
| Author(s): |
Moscoso, Agustin Vintimilla
;
Figueira, Estela Regina Ramos
;
Rocha-Filho, Joel Avancini
;
Urner, Martin
;
Lanchotte, Cinthia
;
Jukemura, Jose
;
Ximenes, Jorge Luiz Saraiva
;
Nahas, Sergio Carlos
;
D'Albuquerque, Luiz Augusto Carneiro
;
Galvao, Flavio Henrique Ferreira
Total Authors: 10
|
| Document type: | Journal article |
| Source: | PHARMACOLOGY RESEARCH & PERSPECTIVES; v. 10, n. 6, p. 7-pg., 2022-12-01. |
| Abstract | |
Liver ischemia-reperfusion (IR) injury is associated with poor outcome after liver transplantation and liver resections. Hexafluoroisopropanol (HFIP) is a tri-fluorinated metabolites of volatile anesthetics and has modulatory effects on inflammation that have been observed mainly in cell culture experiments. In this survey, we investigated the effects of HFIP in a rat model of normothermic hepatic ischemia-reperfusion injury. Twenty-four male Wistar rats were randomized into three groups: (1) control in which animals were submitted to 30 min of partial liver ischemia with resection of non-ischemic liver lobes immediate after reperfusion, (2) pre-ischemia (PI) group in which animals received intravenous HFIP (67 mg/kg) 5 min before liver ischemia, and (3) pre-reperfusion (PR) group in which animals received intravenous HFIP (67 mg/kg) 5 min before reperfusion. Four hours after reperfusion, all animals were euthanized for sample collection. Aspartate and alanine transaminases, glucose, and high mobility group box-1 (HMGB-1) protein concentrations showed a significant decreased, and malondialdehyde was increased in the PR group compared with control and PI groups. Interleukin 6 (IL-6) was increased in the PI group compared with control and PR groups. IL-10 and -12 were increased in the PR and PI groups, respectively, when compared with the control group. Glucose decreased in the PR when compared with the control group. Post-conditioning with HFIP led to a decrease in hepatocellular injury and was associated with a downregulation of HMGB-1. The HFIP resulted in a better control of inflammatory response to ischemia-reperfusion even without causing a reduction in oxidative stress. (AU) | |
| FAPESP's process: | 11/05214-3 - Effects of ischemic preconditioning on hepatic hemodynamics and metabolism in an experimental model of liver ischemia/ reperfusion injury in rats |
| Grantee: | Estela Regina Ramos Figueira |
| Support Opportunities: | Regular Research Grants |