| Full text | |
| Author(s): |
Ribeiro, Gabriela
;
Baldi, Fernando
;
Cesar, Aline S. M.
;
Alexandre, Pamela A.
;
Peripolli, Elisa
;
Ferraz, Jose B. S.
;
Fukumasu, Heidge
Total Authors: 7
|
| Document type: | Journal article |
| Source: | BMC Genomics; v. 23, n. 1, p. 14-pg., 2022-11-25. |
| Abstract | |
Background Potential functional variants (PFVs) can be defined as genetic variants responsible for a given phenotype. Ultimately, these are the best DNA markers for animal breeding and selection, especially for polygenic and complex phenotypes. Herein, we described the identification of PFVs for complex phenotypes (in this case, Feed Efficiency in beef cattle) using a systems-biology driven approach based on RNA-seq data from physiologically relevant organs. Results The systems-biology coupled with deep molecular phenotyping by RNA-seq of liver, muscle, hypothalamus, pituitary, and adrenal glands of animals with high and low feed efficiency (FE) measured by residual feed intake (RFI) identified 2,000,936 uniquely variants. Among them, 9986 variants were significantly associated with FE and only 78 had a high impact on protein expression and were considered as PFVs. A set of 169 significant uniquely variants were expressed in all five organs, however, only 27 variants had a moderate impact and none of them a had high impact on protein expression. These results provide evidence of tissue-specific effects of high-impact PFVs. The PFVs were enriched (FDR < 0.05) for processing and presentation of MHC Class I and II mediated antigens, which are an important part of the adaptive immune response. The experimental validation of these PFVs was demonstrated by the increased prediction accuracy for RFI using the weighted G matrix (ssGBLUP+wG; Acc = 0.10 and b = 0.48) obtained in the ssGWAS in comparison to the unweighted G matrix (ssGBLUP; Acc = 0.29 and b = 1.10). Conclusion Here we identified PFVs for FE in beef cattle using a strategy based on systems-biology and deep molecular phenotyping. This approach has great potential to be used in genetic prediction programs, especially for polygenic phenotypes. (AU) | |
| FAPESP's process: | 19/18647-7 - Identification of alternative splicing regulators associated with feed efficiency in cattle |
| Grantee: | Gabriela Ribeiro |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |
| FAPESP's process: | 19/01234-1 - Identification of functional variants of the type SNP, indels and isoforms of alternative splicing associated with feed efficiency in Nelore cattle |
| Grantee: | Gabriela Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Master |