Interaction between Nanoparticles, Membranes and Proteins: A Surface Plasmon Resonance Study

Author(s)

de Macedo, Erenildo Ferreira ; Santos, Nivia Salles ; Nascimento, Lucca Silva ; Mathey, Raphael ; Brenet, Sophie ; de Moura, Matheus Sacilotto ; Hou, Yanxia ; Tada, Dayane Batista

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 1, p. 20-pg., 2023-01-01.

Abstract

Regardless of the promising use of nanoparticles (NPs) in biomedical applications, several toxic effects have increased the concerns about the safety of these nanomaterials. Although the pathways for NPs toxicity are diverse and dependent upon many parameters such as the nature of the nanoparticle and the biochemical environment, numerous studies have provided evidence that direct contact between NPs and biomolecules or cell membranes leads to cell inactivation or damage and may be a primary mechanism for cytotoxicity. In such a context, this work focused on developing a fast and accurate method to characterize the interaction between NPs, proteins and lipidic membranes by surface plasmon resonance imaging (SPRi) technique. The interaction of gold NPs with mimetic membranes was evaluated by monitoring the variation of reflectivity after several consecutive gold NPs injections on the lipidic membranes prepared on the SPRi biochip. The interaction on the membranes with varied lipidic composition was compared regarding the total surface concentration density of gold NPs adsorbed on them. Then, the interaction of gold and silver NPs with blood proteins was analyzed regarding their kinetic profile of the association/dissociation and dissociation constants (k(off)). The surface concentration density on the membrane composed of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine and cholesterol (POPC/cholesterol) was 2.5 times higher than the value found after the injections of gold NPs on POPC only or with dimethyldioctadecylammonium (POPC/DDAB). Regarding the proteins, gold NPs showed preferential binding to fibrinogen resulting in a value of the variation of reflectivity that was 8 times higher than the value found for the other proteins. Differently, silver NPs showed similar interaction on all the tested proteins but with a variation of reflectivity on immunoglobulin G (IgG) 2 times higher than the value found for the other tested proteins. (AU)

• FAPESP's process: 17/01697-6 - Synthesis and functionalization of bimetallic nanoparticles for application in photodynamic therapy of cancer
• Grantee: Dayane Batista Tada
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/06803-4 - Development of biosensor for ovarian cancer biomarkers based on SPRI technique
• Grantee: Erenildo Ferreira de Macedo
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/14795-3 - SPRI studies on the affinity between metallic nanoparticles and proteins from human serum
• Grantee: Nivia Salles Santos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/07354-0 - Development of biosensor for Ovarian Cancer biomarkers based on surface plasmon resonance imaging
• Grantee: Erenildo Ferreira de Macedo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/00807-5 - The use of bimetallic nanoparticles for the simultabeous detection of proteins in a multicomponent solution by surface plasmon resonance
• Grantee: Lucca Silva Nascimento
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/20503-5 - Study of the interaction between nanoparticles and proteins by surface plasmon resonance imaging
• Grantee: Nivia Salles Santos
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation