| Full text | |
| Author(s): |
Vecchi, Lara
;
Soares Mota, Sara Teixeira
;
Pereira Zoia, Mariana Alves
;
Martins, Isabella Castro
;
de Souza, Jessica Brito
;
Santos, Tiago Goss
;
Beserra, Adriano de Oliveira
;
de Andrade, Victor Piana
;
Goulart, Luiz Ricardo
;
Araujo, Thaise Goncalves
Total Authors: 10
|
| Document type: | Journal article |
| Source: | CELLS; v. 11, n. 10, p. 22-pg., 2022-05-01. |
| Abstract | |
Annexin A1 (AnxA1) is a pleiotropic protein that exerts essential roles in breast cancer (BC) growth and aggressiveness. In our previous work, we described the autocrine signaling of AnxA1 through formyl peptide receptor 1 (FPR1) in the triple-negative (TN) BC cell line, MDA-MB-231. Here, we aimed to describe the interaction between the AnxA1/FPR1 and the Interleukin-6 (IL-6) signaling pathways and their role in the tumor microenvironment (TME). First, we demonstrated that AnxA1 and IL-6 expression levels are correlated in BC tissue samples. In three TNBC cell lines, overexpression of both AnxA1 and IL-6 was also identified. Next, we inhibited FPR1, the IL-6 receptor and STAT3 in both MDA-MB-231 and MDA-MB-157 cells. The FPR1 inhibition led to increased levels of IL-6 and secreted AnxA1 in both cell lines. On the other side, inhibition of the IL-6 receptor or STAT3 led to the impairment of AnxA1 secretion, suggesting the essential role of the IL-6 signaling cascade in the activation of the AnxA1/FPR1 autocrine axis. Finally, we described the interaction between IL-6 and the AnxA1/FPR1 pathways and their role on the TME by analyzing the effect of supernatants derived from MDA-MB-231 and MDA-MB-157 cells under the inhibition of FPR1 or IL-6 signaling on fibroblast cell motility. (AU) | |
| FAPESP's process: | 09/14027-2 - Mechanisms associated with the function of prion protein and its ligand STI1/Hop: therapeutic approaches |
| Grantee: | Vilma Regina Martins |
| Support Opportunities: | Research Projects - Thematic Grants |