Species-specific molecular differentiation of embryonic inner cell mass and trophectoderm: A systematic review

Author(s)

Marsico, Thamiris Vieira ; Valente, Roniele Santana ; Annes, Kelly ; Oliveira, Andressa Minozzo ; Silva, Mara Viana ; Sudano, Mateus Jose

• Document type: Journal article
• Source: Animal Reproduction Science; v. 252, p. 11-pg., 2023-04-18.

Abstract

A wide-ranging review study regarding the molecular characterization of the first cell lineages of the developmental embryo is lacking, especially for the primary events during earliest differentiation which leads to the determination of cellular fate. Here, a systematic review and meta-analysis were conducted according to PRISMA guidelines. MEDLINE-PubMed was searched based on an established search strategy through April 2021. Thirty-six studies fulfilling the inclusion criteria were subjected to qualitative and quantitative analysis. Among the studies, 50 % (18/36) used mice as an animal model, 22.2 % (8/36) pigs, 16.7 % (6/36) cattle, 5.5 % (2/36) humans, and 2.8 % (1/36) goats as well as 2.8 % (1/36) equine. Our results demonstrated that each of the first cell lineages of embryos requires a certain pattern of expression to establish the cellular determination of fate. Moreover, these patterns are shared by many species, particularly for those molecules that have already been identified in the literature as biomarkers. In conclusion, the present study integrated carefully chosen studies regarding embryonic development and first cellular decisions in mammalian species and summarized the information about the differential characterization of the first cell lineages and their possible relationship with specific gene expression. (AU)

• FAPESP's process: 19/17796-9 - Simultaneous biopsy and lipid profile of embryo inner cell mass and trofectoderm
• Grantee: Thamiris Vieira Marsico
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/21496-8 - Pathways for embryo-derived pregnancy
• Grantee: Mateus José Sudano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 20/08826-9 - Investigation of the cell membrane system and its relationship with cryotolerance and viability of bovine embryos produced in vitro
• Grantee: Kelly Annes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/02139-5 - Transcriptional profiles of embryo competence
• Grantee: Andressa Minozzo Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/18415-9 - Role of inflammatory pathways on embryonic competence and cryotolerance
• Grantee: Roniele Santana Valente
• Support Opportunities: Scholarships in Brazil - Doctorate