Identification of miRnas with possible prognostic roles for HAM/TSP

Author(s)

de Souza, Daniela Raguer Valadao ; Pessoa, Rodrigo ; Nukui, Youko ; Pereira, Juliana ; Marcusso, Rosa Nascimento ; de Oliveira, Augusto Cesar Penalva ; Casseb, Jorge ; Duarte, Alberto Jose da Silva ; Clissa, Patricia Bianca ; Sanabani, Sabri Saeed

• Document type: Journal article
• Source: VIRULENCE; v. 14, n. 1, p. 16-pg., 2023-12-31.

Abstract

Human T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropic spastic paraparesis (HAM/TSP) is an insidiously progressive spinal cord disease for which there is no effective treatment. There is great interest in developing potential biomarkers to predict the pathogenesis of HAM/TSP disease. In this study, Illumina Massive Parallel Sequencing (MPS) technology was used to investigate the cellular global noncoding RNAome expression profile in HAM/TSP patients (n = 10), asymptomatic HTLV-1-infected carriers (ASP, n = 8), and a second group of healthy controls (n = 5). Various bioinformatics tools were used to align, annotate, and profile the sRNA-MPS reads. Among the 402 sRNAs detected, 251 were known and 50 were potentially novel sRNAs in the HAM and ASP groups compared with the HC group. Sixty-eight known sRNAs were significantly different between the ASP and HAM groups. Eighty-eight mature miRNAs were downregulated in subjects from HAM compared with ASP. Three of these miRs (hsa-miR-185-5p, 32-5p, and 192-5p) have the potential to be used as biomarkers for predicting the pathogenesis of HAM/TSP. The seven most deregulated miRs target genes have been associated with a variety of biological processes and molecular functions. The reactome pathways relevant to our findings provide a rich source of data and offer the opportunity to better understand sRNA regulation and function in HTLV-1 pathophysiology. To the best of our knowledge, this study is the first to demonstrate evaluates sRNAs in HTLV-1 patients with HAM/TSP. (AU)

• FAPESP's process: 22/09354-9 - Metabolomic and bacteriome profiling in HAM/TSP patients
• Grantee: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/12297-2 - Profiling the human T-cells miRNA, REX and tax transcriptomes in the course of HTLV-1 infection using a deep sequencing approach
• Grantee: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/24596-2 - HIV massively parallel sequencing data in plasma and peripheral blood mononuclear cells from untreated blood donors: comparison of the near full-length genome subtypes, drug resistance mutations and co-receptor usage
• Grantee: Rodrigo Pessoa de Farias
• Support Opportunities: Scholarships in Brazil - Doctorate