| Full text | |
| Author(s): |
Alexandrino, Andre Vessoni
;
Prieto, Evandro Luis
;
Nicolela, Nicole Castro Silva
;
da Silva Marin, Tamiris Garcia
;
dos Santos, Talita Alves
;
de Oliveira da Silva, Joao Pedro Maia
;
da Cunha, Anderson Ferreira
;
Behlau, Franklin
;
Novo-Mansur, Maria Teresa Marques
Total Authors: 9
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 14, p. 17-pg., 2023-07-01. |
| Abstract | |
Citrus canker, caused by the bacterium Xanthomonas citri (Xcc), is one of the most devastating diseases for the citrus industry. Xylose is a constituent of the cell wall of plants, and the ability of Xcc to use this carbohydrate may play a role in virulence. Xcc has two genes codifying for xylose isomerase (XI), a bifunctional enzyme that interconverts D-xylose into D-xylulose and D-glucose into D-fructose. The aim of this work was to investigate the functional role of the two putative XI ORFs, XAC1776 (xylA1) and XAC4225 (xylA2), in Xcc pathogenicity. XI-coding genes of Xcc were deleted, and the single mutants (Xcc & UDelta;xylA1 or Xcc & UDelta;xylA2) or the double mutant (Xcc & UDelta;xylA1 & UDelta;xylA2) remained viable. The deletion of one or both XI genes (xylA1 and/or xylA2) increased the aggressiveness of the mutants, causing disease symptoms. RT-qPCR analysis of wild strain and xylA deletion mutants grown in vivo and in vitro revealed that the highest expression level of hrpX and xylR was observed in vivo for the double mutant. The results indicate that XI depletion increases the expression of the hrp regulatory genes in Xcc. We concluded that the intracellular accumulation of xylose enhances Xcc virulence. (AU) | |
| FAPESP's process: | 21/14224-4 - Analysis of the xylose isomerase (XylA) and hrp genes expression in Xanthomonas citri subsp. citri in vivo by qRT-PCR and proteomic analysis |
| Grantee: | Evandro Luis Prieto |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 20/05529-3 - Functional analysis of xanB and xylA genes potentially involved with Xanthomonas citri pathogenicity |
| Grantee: | Maria Teresa Marques Novo Mansur |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/17470-0 - Functional analysis of xylose isomerase in the response to xylose by Xanthomonas citri and X. fuscans- type B and differential proteomic analysis of this response |
| Grantee: | Evandro Luis Prieto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |