"Relationship between antibiotic resistance, biofilm formation, virulence factors and source of origin of Pseudomonas aeruginosa environmental isolates with regard to the presence of metallo-β-lactamase-encoding genes"

Author(s):Show less -

Gervasoni, Leticia Franco ; Peixoto, Inaia Calegari ; Imperador, Anna Cardoso ; De Oliveira, Livia Batista ; Correia, Lucimeire Fernandes ; Vieira, Karolinny Cristiny de Oliveira ; Saeki, Erika Kushikawa ; Lima, Paula Esteves da Silva ; Mareco, Edson Assuncao ; Pereira, Valeria Cataneli ; Eller, Lizziane Kretli Winkelstroter

• Document type: Journal article
• Source: Microbial Pathogenesis; v. 182, p. 8-pg., 2023-07-09.

Abstract

A total of 557 water samples were evaluated and of these, 23 were positive for the presence of Pseudomonas aeruginosa. Approximately 91.7% of them were weak biofilm formers. Only 4 isolates showed antimicrobial resistance. All isolates presented Twitching motility, a positive result for the production of pyocyanin, alkaline protease, and hemolysins. The genotypic tests showed: lasA, (95.6%) lasB (95.6%), exoS (95.6%), exoT (91.3%), toxA (91.3%), akgO (91.3%), plcN (91.3%) aprA (86.9%), phzM (78.3%), and pvdA (60.9%). For genes encoding metallo-beta-lactamase, it was found: blaVIM (56.6%), blaSPM (4.3%), and blaSIM (47.8%). A strong association was found between the metallo-beta-lactamase producing genes, nine genes of virulence factors and the motility (r = 0.6231). The very close clonal profile suggests a probable similarity between the isolates from different cities. Thus, P. aeruginosa can be present in water supplies with variable virulence capacities and can generate a huge concern for human, animal, and environmental health. (AU)

• FAPESP's process: 20/08262-8 - Occurrence and characterization of virulence and antimicrobial resistance factors of Pseudomonas aeruginosa isolated from water samples from Western São Paulo
• Grantee: Letícia Franco Gervasoni
• Support Opportunities: Scholarships in Brazil - Scientific Initiation