| Full text | |
| Author(s): Show less - |
Nascimento, Janaina F.
;
Souza, Rodolpho O. O.
;
Alencar, Mayke B.
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Marsiccobetre, Sabrina
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Murillo, Ana M.
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Damasceno, Flavia S.
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Girard, Richard B. M. M.
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Marchese, Leticia
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Luevano-Martinez, Luis A.
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Achjian, Renan W.
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Haanstra, Jurgen R.
;
Michels, Paul A. M.
;
Silber, Ariel M.
Total Authors: 13
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| Document type: | Journal article |
| Source: | PLOS PATHOGENS; v. 19, n. 7, p. 35-pg., 2023-07-01. |
| Abstract | |
ATP hydrolysis is required for the synthesis, transport and polymerization of monomers for macromolecules as well as for the assembly of the latter into cellular structures. Other cellular processes not directly related to synthesis of biomass, such as maintenance of membrane potential and cellular shape, also require ATP. The unicellular flagellated parasite Trypanosoma brucei has a complex digenetic life cycle. The primary energy source for this parasite in its bloodstream form (BSF) is glucose, which is abundant in the host's bloodstream. Here, we made a detailed estimation of the energy budget during the BSF cell cycle. As glycolysis is the source of most produced ATP, we calculated that a single parasite produces 6.0 x 10(11) molecules of ATP/cell cycle. Total biomass production (which involves biomass maintenance and duplication) accounts for similar to 63% of the total energy budget, while the total biomass duplication accounts for the remaining similar to 37% of the ATP consumption, with in both cases translation being the most expensive process. These values allowed us to estimate a theoretical Y-ATP of 10.1 (g biomass)/mole ATP and a theoretical Y-ATP(max) of 28.6 (g biomass)/mole ATP. Flagellar motility, variant surface glycoprotein recycling, transport and maintenance of transmembrane potential account for less than 30% of the consumed ATP. Finally, there is still similar to 5.5% available in the budget that is being used for other cellular processes of as yet unknown cost. These data put a new perspective on the assumptions about the relative energetic weight of the processes a BSF trypanosome undergoes during its cell cycle. (AU) | |
| FAPESP's process: | 21/12938-0 - Amino acid metabolism in Trypanosoma cruzi: a toolbox to survive in hostile environments |
| Grantee: | Ariel Mariano Silber |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/14432-3 - A network for an integrative biology in neglected diseases: bridging epigenetics, metabolism and cell cycle in pathogenic trypanosomatids |
| Grantee: | Ariel Mariano Silber |
| Support Opportunities: | Research Projects - Thematic Grants |