| Full text | |
| Author(s): |
Bibiano, Lillian B. J.
;
Galesi, Ana Luiza H.
;
Dumit, Amanda G.
;
Carvalho, Camila P.
;
Gioria, Ricardo
;
Rezende, Jorge A. M.
;
Camargo, Luis E. A.
Total Authors: 7
|
| Document type: | Journal article |
| Source: | EUPHYTICA; v. 219, n. 9, p. 9-pg., 2023-09-01. |
| Abstract | |
The melon accession PI414723 carries the Zym-1 resistance locus, which is used in breeding programs to control the disease caused by zucchini yellow mosaic virus (ZYMV). We investigated the effects of Zym-1 on the phenotype and on viral titers of F-2 plants derived from the cross between PI414723 and Vedrantais. For that, we used the simple sequence repeat marker CMAG36, which is linked to Zym-1, and a developed quantitative PCR (qPCR) protocol based on the coat protein of ZYMV. PI414723 and F1 remained symptomless 21 days after inoculation (dai), whereas Vedrantais displayed extreme susceptibility. At 8 dai ZYMV was not detected in the apical leaf of the resistant genotype but was in F-1 plants at very low levels. Most plants homozygous for resistance (R) or susceptibility (S) alleles at Zym-1 were either symptomless or presented extreme symptoms, respectively. However, a few opposite cases were observed in the two genotypes. Different from the R and F1 plants, the reactions of heterozygotes (H) were more diverse, indicating a partial dominance of Zym-1 at the phenotypic level. ZYMV was detected on R and H plants in equivalent titers, but about 59 times lower than in S plants. This study illustrates the major effects of Zym-1 on the phenotype and viral multiplication or movement. It indicates that in the heterozygous state, Zym-1 may interact with the other resistance genes of PI414723 in an epistatic manner. (AU) | |
| FAPESP's process: | 19/13397-2 - Genetics and genomics applied to the identification of the Zym-1 and Zym-2 resistance genes of melon against Zucchini yellow mosaic virus |
| Grantee: | Luis Eduardo Aranha Camargo |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 20/13481-0 - Increased genetic resolution of the Zym-2 locus of resistance to Zucchini yellow mosaic virus (ZYMV) in melon with microsatellite markers and identification of candidate genes |
| Grantee: | Amanda Ghelfi Dumit |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |