Association of single nucleotide variants in VEGFA and KDR with the risk and angiogenic features of diffuse large B-cell lymphoma

Author(s)

Assis-Mendonca, Guilherme Rossi ; Campos, Leticia Goulart ; Delamain, Marcia Torresan ; de Brito, Angelo Borsarelli Carvalho ; Fanelli, Marcello Ferreti ; Soares, Fernando Augusto ; de Souza, Carmino Antonio ; Vassallo, Jose ; Lima, Carmen Silvia Passos

• Document type: Journal article
• Source: Leukemia & Lymphoma; v. N/A, p. 13-pg., 2023-08-29.

Abstract

Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma subtype and dependent on angiogenesis (AG), whose main effectors are VEGFA and VEGFR2. Functional single nucleotide variants (SNVs) are described in VEGFA and KDR genes. However, it still unknown whether VEGFA - 2578C/A, -2489C/T, -1154G/A, -634G/C, -460C/T and KDR-604T/C, -271G/A, +1192G/A and +1719A/T SNVs act on DLBCL risk and angiogenic features. Genomic DNA from 168 DLBCL patients and 205 controls was used for SNV genotyping. Angiogenesis was immunohistochemically assessed in tumor biopsies, with reactions for VEGFA, VEGFR2, and CD34. VEGFA -1154GG genotype were associated with 1.6-fold higher DLBCL risk. KDR + 1192GG plus KDR + 1719 TT and KDR + 1192GG plus VEGFA - 2578CC combined genotypes are associated with 2.19- and 2.04-fold higher risks of DLBCL, respectively. VEGFA - 634GG or GC genotypes are associated with increased microvessel density and VEGFA levels. No relationship was observed between SNVs and cell-of-origin classification of DLBCL, but higher VEGFA and VEGFR2 were seen in non-germinal center tumors. (AU)

• FAPESP's process: 15/15756-9 - The role of polymorphisms in VEGF and VEGFR2 genes, related to angiogenesis, in clinical features, and prognosis of Diffuse Large B Cell Lymphoma patients
• Grantee: Angelo Borsarelli Carvalho de Brito
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/09854-5 - Tumor microenvironment in indolent non-Hodgkin lymphomas: role of tumoral microRNAs and inflammatory polymorphisms in clinical and pathological aspects
• Grantee: José Vassallo
• Support Opportunities: Regular Research Grants