| Full text | |
| Author(s): Show less - |
Leo, Tiago F.
;
Wang, Mingxun
;
da Silva, Ricardo
;
Gurevich, Alexey
;
Bauermeister, Anelize
;
Gomes, Paulo Wender P.
;
Brejnrod, Asker
;
Glukhov, Evgenia
;
Aron, Allegra T.
;
Louwen, Joris J. R.
;
Kim, Hyun Woo
;
Reher, Raphael
;
Fiore, Marli F.
;
van der Hooft, Justin J. J.
;
Gerwick, Lena
;
Gerwick, William H.
;
Bandeira, Nuno
;
Dorrestein, Pieter C.
Total Authors: 18
|
| Document type: | Journal article |
| Source: | PNAS NEXUS; v. 1, n. 5, p. 15-pg., 2022-11-01. |
| Abstract | |
Microbial specialized metabolites are an important source of and inspiration for many pharmaceuticals, biotechnological products and play key roles in ecological processes. Untargeted metabolomics using liquid chromatography coupled with tandem mass spectrometry is an efficient technique to access metabolites from fractions and even environmental crude extracts. Nevertheless, metabolomics is limited in predicting structures or bioactivities for cryptic metabolites. Efficiently linking the biosynthetic potential inferred from (meta)genomics to the specialized metabolome would accelerate drug discovery programs by allowing metabolomics to make use of genetic predictions. Here, we present a k-nearest neighbor classifier to systematically connect mass spectrometry fragmentation spectra to their corresponding biosynthetic gene clusters (independent of their chemical class). Our new pattern-based genome mining pipeline links biosynthetic genes to metabolites that they encode for, as detected via mass spectrometry from bacterial cultures or environmental microbiomes. Using paired datasets that include validated genes-mass spectral links from the Paired Omics Data Platform, we demonstrate this approach by automatically linking 18 previously known mass spectra (17 for which the biosynthesis gene clusters can be found at the MIBiG database plus palmyramide A) to their corresponding previously experimentally validated biosynthetic genes (e.g., via nuclear magnetic resonance or genetic engineering). We illustrated a computational example of how to use our Natural Products Mixed Omics (NPOmix) tool for siderophore mining that can be reproduced by the users. We conclude that NPOmix minimizes the need for culturing (it worked well on microbiomes) and facilitates specialized metabolite prioritization based on integrative omics mining. (AU) | |
| FAPESP's process: | 17/18922-2 - Development of a computing platform extensible and modular for metabolomics and metagenomics analysis: innovation with the discovery of new enzymatic activities and natural products of pharmaceutical interest derived |
| Grantee: | Ricardo Roberto da Silva |
| Support Opportunities: | BIOTA-FAPESP Program - Young Investigators Grants |
| FAPESP's process: | 19/05026-4 - Development of a computational platform extensible and modular for analysis of metabolomics and metagenomics experiments: Innovating with the discovery of new enzymatic activities and derived natural products of interest |
| Grantee: | Ricardo Roberto da Silva |
| Support Opportunities: | Scholarships in Brazil - BIOTA - Young Researchers |