| Full text | |
| Author(s): |
Queiroz, Joao Paulo da Silva
;
Pupin, Breno
;
Bhattacharjee, Tanmoy Tapobrata
;
Uno, Miyuki
;
Chammas, Roger
;
Kulcsar, Marco Aurelio Vamondes
;
Canevari, Renata de Azevedo
Total Authors: 7
|
| Document type: | Journal article |
| Source: | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 304, p. 8-pg., 2024-01-05. |
| Abstract | |
We explore the feasibility of using FOS and JUN gene expression and ATR-FTIR for diagnosis of thyroid cancer. For the study, 38 samples (6 non-neoplastic (NN), 10 papillary thyroid carcinoma (PTC), 7 follicular thyroid carcinoma (FTC), and 15 benign tumors (BT) were subjected to RNA extraction followed by quantitative real time PCR (qRT-PCR) and 30 samples (5 NN, 9 PTC, 5 FTC, and 11 BT) were used for Attenuated Total Reflectance - Fourier Transform Infrared (ATR-FTIR) followed by multivariate analysis. Of the above, 20 samples were used for both gene expression and ATR-FTIR studies. We found FOS and JUN expression in malignant tumor samples to be significantly lower than NN and benign. ATR-FIR after multivariate analysis could identify the difficult to diagnose FTC with 93 % efficiency. Overall, results suggest the diagnostic potential of molecular biology techniques combined with ATR-FTIR spectroscopy in differentiated thyroid carcinomas (PTC and FTC) and BT. (AU) | |
| FAPESP's process: | 19/10211-5 - Evaluation of the diagnostic potential of genes belonging to the MAP/KINASE pathway in thyroid lesions |
| Grantee: | João Paulo da Silva Queiroz |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 09/18440-1 - Development of molecular markers related to cellular resistance after photodynamic therapy in breast carcinomas |
| Grantee: | Renata de Azevedo Canevari |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/14464-9 - Molecular and biochemical characterization of thyroid carcinomas for clinical diagnosis |
| Grantee: | Renata de Azevedo Canevari |
| Support Opportunities: | Regular Research Grants |