Expression data of FOS and JUN genes and FTIR spectra provide diagnosis of thyroid carcinoma

Author(s)

Queiroz, Joao Paulo da Silva ; Pupin, Breno ; Bhattacharjee, Tanmoy Tapobrata ; Uno, Miyuki ; Chammas, Roger ; Kulcsar, Marco Aurelio Vamondes ; Canevari, Renata de Azevedo

• Document type: Journal article
• Source: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 304, p. 8-pg., 2024-01-05.

Abstract

We explore the feasibility of using FOS and JUN gene expression and ATR-FTIR for diagnosis of thyroid cancer. For the study, 38 samples (6 non-neoplastic (NN), 10 papillary thyroid carcinoma (PTC), 7 follicular thyroid carcinoma (FTC), and 15 benign tumors (BT) were subjected to RNA extraction followed by quantitative real time PCR (qRT-PCR) and 30 samples (5 NN, 9 PTC, 5 FTC, and 11 BT) were used for Attenuated Total Reflectance - Fourier Transform Infrared (ATR-FTIR) followed by multivariate analysis. Of the above, 20 samples were used for both gene expression and ATR-FTIR studies. We found FOS and JUN expression in malignant tumor samples to be significantly lower than NN and benign. ATR-FIR after multivariate analysis could identify the difficult to diagnose FTC with 93 % efficiency. Overall, results suggest the diagnostic potential of molecular biology techniques combined with ATR-FTIR spectroscopy in differentiated thyroid carcinomas (PTC and FTC) and BT. (AU)

• FAPESP's process: 19/10211-5 - Evaluation of the diagnostic potential of genes belonging to the MAP/KINASE pathway in thyroid lesions
• Grantee: João Paulo da Silva Queiroz
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 09/18440-1 - Development of molecular markers related to cellular resistance after photodynamic therapy in breast carcinomas
• Grantee: Renata de Azevedo Canevari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14464-9 - Molecular and biochemical characterization of thyroid carcinomas for clinical diagnosis
• Grantee: Renata de Azevedo Canevari
• Support Opportunities: Regular Research Grants