Protocol for design, construction, and selection of genome phage (gPhage) display libraries

Author(s):Show less -

Carnero, Luis Antonio Rodriguez ; Reis, AndreAzevedo ; Tang, Fenny Hui Fen ; Kuramoto, Andreia ; Pasqualini, Renata ; Arap, Wadih ; Setubal, Joao Carlos ; Cunha-Neto, Edecio ; JoseGiordano, Ricardo ; Alves, Maria Julia Manso ; Colli, Walter

• Document type: Journal article
• Source: STAR PROTOCOLS; v. 2, n. 4, p. 24-pg., 2021-12-17.

Abstract

This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns.For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021). (AU)

• FAPESP's process: 14/21177-9 - Identification of molecular markers for the development of novel therapeutic agents with angiogenic properties
• Grantee: Ricardo Jose Giordano
• Support Opportunities: Regular Research Grants