| Full text | |
| Author(s): |
Neto, Dionisio Pedro Amorim
;
Fonseca, Matheus de Castro
Total Authors: 2
|
| Document type: | Journal article |
| Source: | STAR PROTOCOLS; v. 3, n. 3, p. 11-pg., 2022-09-16. |
| Abstract | |
Gut microbiota influences neurodevelopment, behavior and contributes to neurodegenerative disorders. One possible mechanism is the direct modulation of calcium (Ca2+) signaling and protein homeostasis in enteroendocrine cells (EECs), a component of the gut epithelium. Here, we present a protocol to isolate fractions of conditioned media (CM) from the anaerobic bacteria Akkermansia muciniphila and the utilization of this CM to monitor Ca2+ fluctuation in EECs by imaging. This protocol can be adapted and applied to various bacterial cultures and cell types.For complete details on the use and execution of this protocol, please refer to Amorim Neto et al. (2022). (AU) | |
| FAPESP's process: | 19/24511-0 - The role of LRRK2 and Rab GTPases in the neurodegeneration during sporadic Parkinson's Disease |
| Grantee: | Beatriz Pelegrini Bosque |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/20014-0 - The gut-brain connection: role of the gut microbioma in the outcome and development of the sporadic Parkinson's disease |
| Grantee: | Matheus de Castro Fonseca |
| Support Opportunities: | Regular Research Grants |