Effect of Kinins on the Hepatic Oxidative Stress in Mice Treated with a Methionine-Choline Deficient Diet

Author(s)

Thomaz, Mariana Silva ; Sertorio, Marcela Nascimento ; Gazarini, Marcos Leoni ; Ribeiro, Daniel Araki ; Pisani, Luciana Pellegrini ; Nagaoka, Marcia Regina

• Document type: Journal article
• Source: BIOMEDICINES; v. 11, n. 8, p. 15-pg., 2023-08-01.

Abstract

Non-alcoholic fatty liver is the leading cause of hepatic disease worldwide and ranges from simple steatosis to non-alcoholic steatohepatitis (NASH) due to cell injury, oxidative stress, and apoptosis. The kinins' role in the liver has been studied in experimental fibrosis, partial hepatectomy, and ischemia-reperfusion and is related to cell death and regeneration. We investigated its role in experimental NASH induced by a methionine-choline deficient diet for 4 weeks. After that, liver perfusion was performed, and bradykinin (BK) or des-Arg(9)-BK was infused. Cell death was evaluated by cathepsin-B and caspase-3 activity and oxidative stress by catalase (CAT), glutathione S-transferase, and superoxide dismutase (SOD) activities, as well as malondialdehyde and carbonylated proteins. In control livers, DABK increased CAT activity, which was reversed by antagonist DALBK. In the NASH group, kinins tend to decrease antioxidant activity, with SOD activity being significantly reduced by BK and DABK. Malondialdehyde levels increased in all NASH groups, but carbonylated protein did not. DABK significantly decreased cathepsin-B in the NASH group, while caspase-3 was increased by BK in control animals. Our results suggest that B1R and/or B2R activation did not induce oxidative stress but affected the antioxidant system, reducing SOD in the NASH group. (AU)

• FAPESP's process: 17/00896-5 - Hepatic cytoprotection: from fibrosis to ischemia and reperfusion
• Grantee: Márcia Regina Nagaoka
• Support Opportunities: Regular Research Grants