| Full text | |
| Author(s): Show less - |
da Silva, Victor A.
;
Bobotis, Bianca C.
;
Correia, Felipe F.
;
Lima-Vasconcellos, Theo H.
;
Chiarantin, Gabrielly M. D.
;
De la Vega, Laura
;
Lombello, Christiane B.
;
Willerth, Stephanie M.
;
Malmonge, Sonia M.
;
Paschon, Vera
;
Kihara, Alexandre H.
Total Authors: 11
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 17, p. 25-pg., 2023-09-01. |
| Abstract | |
Tissue engineering for spinal cord injury (SCI) remains a complex and challenging task. Biomaterial scaffolds have been suggested as a potential solution for supporting cell survival and differentiation at the injury site. However, different biomaterials display multiple properties that significantly impact neural tissue at a cellular level. Here, we evaluated the behavior of different cell lines seeded on chitosan (CHI), poly (& epsilon;-caprolactone) (PCL), and poly (L-lactic acid) (PLLA) scaffolds. We demonstrated that the surface properties of a material play a crucial role in cell morphology and differentiation. While the direct contact of a polymer with the cells did not cause cytotoxicity or inhibit the spread of neural progenitor cells derived from neurospheres (NPCdn), neonatal rat spinal cord cells (SCC) and NPCdn only attached and matured on PCL and PLLA surfaces. Scanning electron microscopy and computational analysis suggested that cells attached to the material's surface emerged into distinct morphological populations. Flow cytometry revealed a higher differentiation of neural progenitor cells derived from human induced pluripotent stem cells (hiPSC-NPC) into glial cells on all biomaterials. Immunofluorescence assays demonstrated that PCL and PLLA guided neuronal differentiation and network development in SCC. Our data emphasize the importance of selecting appropriate biomaterials for tissue engineering in SCI treatment. (AU) | |
| FAPESP's process: | 17/12112-9 - EZH2 cloning and retroviral preparation for lens primary cell culture transfection |
| Grantee: | Felipe Fernandes Correia |
| Support Opportunities: | Scholarships abroad - Research Internship - Scientific Initiation |
| FAPESP's process: | 18/06316-3 - Functional consequences of EZH2 epigenetical regulation on neurodegeneration and locomotor recovery after spinal cord compression |
| Grantee: | Felipe Fernandes Correia |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/19300-8 - Evaluation and characterization of neural differentiation in polymeric scaffolds for medular regeneration |
| Grantee: | Victor Allisson da Silva |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 19/17892-8 - Degeneration and development of the nervous system: the role of epigenetic processes |
| Grantee: | Alexandre Hiroaki Kihara |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/18921-1 - The role of EZH2 on glial transcriptional response and motricity recovery after spinal cord injury |
| Grantee: | Felipe Fernandes Correia |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |
| FAPESP's process: | 20/02035-0 - Descriptive and functional analysis of the IP3R1 receptor in the pathology of retinitis pigmentosa using C3H/HeJ mice model |
| Grantee: | Théo Henrique de Lima Vasconcellos |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 20/11667-0 - Cellular and electrophysiological changes in the hippocampus caused by neurodegenerative processes related to neonatal anoxia and temporal lobe epilepsy |
| Grantee: | Alexandre Hiroaki Kihara |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 21/11969-9 - The role of IP3R1 in neuroinflammation triggered by retinitis pigmentosa |
| Grantee: | Théo Henrique de Lima Vasconcellos |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 19/24406-2 - Evaluation of the impact of EZH2-related epigenetic changes on polarization and proliferation of spinal cord microglia in vitro |
| Grantee: | Bianca Caroline Bobotis |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |