| Full text | |
| Author(s): |
Goulart, Herbert Rodrigues
;
Arthuso, Fernanda dos Santos
;
Nucci Capone, Marcos Vinicius
;
de Oliveira, Tais Lima
;
Bartolini, Paolo
;
Jorge Soares, Carlos Roberto
Total Authors: 6
|
| Document type: | Journal article |
| Source: | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY; v. N/A, p. 11-pg., 2010-01-01. |
| Abstract | |
Sodium butyrate (NaBu) has been used as a productivity enhancer for the synthesis of recombinant proteins in Chinese hamster ovary (CHO) cells. Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was investigated for the first time. CHO cell cultures were submitted to a treatment with different concentrations of NaBu (0.25 to 4 mM). Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC) and Western blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained with 1 mM NaBu. In vitro biological assays based on noble rat lymphoma (Nb2) and mouse pro-B lymphoma (Ba/F3-LLP) cells were carried out on purified hPRL. Its bioactivity in the presence of NaBu was not apparently different from that of the First International Reference Reagent of recombinant hPRL (WHO 97/714). Our results show that NaBu increased the synthesis of recombinant hPRL in CHO cells, apparently without compromising either its structure or function. (AU) | |
| FAPESP's process: | 07/59709-8 - Sintese e caracterizacao de prolactina de camundongo (mprl) em escherichia coli. |
| Grantee: | Fernanda dos Santos Arthuso |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 07/54493-7 - Inibição do efeito pró-apoptótico do butirato de sódio: um método alternativo para incrementar a biossíntese de prolactina e de outros hormônios hipofisários humanos em células CHO |
| Grantee: | Herbert Rodrigues Goulart |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 07/59540-3 - Sintese e caracterizacao de prolactina de camundongos (mprl) e de seu analogo (s177d-mprl) em escherichia coli. |
| Grantee: | Carlos Roberto Jorge Soares |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 05/60826-3 - Desenvolvimento de bioprocesso para producao de larga escala de hormonios recombinantes e seus antagonistas em cultura de celulas cho em biorreatores. |
| Grantee: | Carlos Roberto Jorge Soares |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 06/52973-9 - Sintese, purificacao e caracterizacao da prolactina humana glicosilada produzida em celulas de ovario de hamster chines. |
| Grantee: | Susana da Rocha Heller |
| Support Opportunities: | Scholarships in Brazil - Master |