Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil

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Author(s):	Chavez, Juliana Helena ; dos Reis, Vinicius Pinho ; Silva, Jaqueline Raymondi ; Laure, Helen Julie ; Rosa, Jose Cesar ; Lopes da Fonseca, Benedito Antonio ; Moraes Figueiredo, Luiz Tadeu Total Authors: 7
Document type:	Journal article
Source:	Revista da Sociedade Brasileira de Medicina Tropical; v. 46, n. 1, p. 3-pg., 2013-01-01.
Abstract
Introduction: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. Methods: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. Results: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. Conclusions: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil. (AU)